85results about How to "Sampling safety" patented technology

The invention belongs to the field of oil gas and oil well development, discloses a system for sampling and monitoring components of shaft fluid of a gas field and particularly relates to a system for sampling and monitoring components of the shaft fluid of the gas field and a sampling and analysis method thereof, which are designed aiming at environmental characteristics of a H2S/CO2-containing gas field. A sampling process of a sampler of a gas well is safer, an experimental process is more accurate and quicker, and the application pressure range is wider and safer, and an experimental result of researching the change of high-content hydrogen sulfide gas components is more accurate; and a device is safe and reliable. Through the system and the method, the problems that fluid pressure cannot be obtained and fluid deposit in the shaft cannot be predicted because the measurement cannot be performed in the gas field containing high content of H2S/CO2 can be solved and theoretical basis is provided for a gas well capacity design, production parameter optimization and the formulation of technical measures.

The invention belongs to the field of development of oil and gas wells, and relates to a wellhead sampling device, in particular to a wellhead sampler designed for the characteristic of gas field environment containing high content of H2S/CO2, and a sampling system thereof. The sampling device comprises a sampling valve, a sampling bottle and a pressure reducing valve, wherein two ends of the sampling bottle are in threaded connection with the sampling valve; the external connection of the sampling valve is external-thread clamping sleeve type connection; the pressure reducing valve is used for carrying out pressure reduction and sample injection on high-pressure gas in the sampling bottle; the pressure reducing valve comprises an outer conveying sample injection port which is connected with an outer detector; and the outer conveying sample injection port is a clamping sleeve type connection port so as to eliminate dead angles of pipelines. In the invention, the field sampling for analysis is achieved, the result of experimental analysis has good consistency, the device is safe and reliable, and the corrosion resistance to H2S/CO2 is strong. In the gas field sampler disclosed by the invention, the sampling process is safer, the experimental process is more precise and faster, the applicable pressure range is wider, and more safety is achieved.

The invention discloses a method for detecting and analyzing a biomembrane on the inner wall of an oil field sewage pipe. In the invention, an inner ball valve arranged on the inner wall of the oil field sewage pipe is used for controlling; a protecting sleeve is in threaded connection with the ball valve; a sealing ring at the inner periphery of the protecting sleeve presses on a seal and is in threaded connection with a pressing cap with the protecting sleeve; a positioning screw arranged on the pressing cap passes through the pressing cap, the protecting sleeve and a sampler, wherein the sampler consists of a sampling rod inserted into the inner wall of the oil field sewage pipe in the center of the ball valve and a test sheet carrier arranged at the lower end of the sampling rod; a test sheet which is made from the same material and is used as the sewage pipe is hung on the inner wall of the oil field sewage pipe; and the biomembrane of the sewage pipe is detected through the test sheet. The period for detecting the biomembrane is over 14 days. The period for detecting the biomembrane can be prolonged according to the requirement on the thickness of the biomembrane. The detected biomembrane is analyzed and detected. Bacterium amount detection, strain identification total biomass detection and the detection on the activity inhabitation ability of bactericides on bacteria in the biomembrane are respectively performed on the detected biomembrane, and an adaptive bactericide is selected from the bactericides, so the aims of good sterilizing effect, small bactericide using amount and sewage treatment cost conservation are fulfilled.

The invention discloses a method for the multistage sampling of a floating-roof oil tank. The method comprises the following steps of: designing an upper part sampling pipe, an upper middle part sampling pipe, a middle part sampling pipe, a lower middle part sampling pipe and a lower part sampling pipe from the upper part, the upper middle part, the middle part, the lower middle part and the lower part of the circumferential side of a central drainage pipe of the floating-roof oil tank to the outside of the lower side of the same circumferential side of the floating-roof oil tank; connecting the distal ends of the sampling pipes in parallel and then connecting with an inlet of a gear pump, and respectively designing a sampling control valve at the pipelines before being connected in parallel; connecting an outlet of the gear pump with the lower part of the floating-roof oil tank by an oil return pipe, and designing a gear pump outlet control valve and a sampling valve at the front part and the back part of the oil return pipe; and opening a gear pump inlet control valve and the gear pump outlet control valve of the upper part sampling pipe when sampling oil of the upper part of the floating-roof oil tank, starting the gear pump to firstly drain the dead oil in the sampling pipes into the floating-roof oil tank through the oil return pipe, and then opening the sampling valves to sample, wherein the sampling process of each part is same. The method of the invention is convenient to the multistage sampling of the floating-roof oil tank and also saves time and labor.

The invention discloses a hydrogeological water quality sampling device and a using method thereof. The device comprises a base, a sampling pipe and an electromagnetic valve, wherein the sampling pipeis connected to the upper end of the base through a clamping groove, a water inlet is formed in one side wall of the sampling pipe, the electromagnetic valve is connected to the water inlet through apipe hoop, and a cover plate is connected to the upper end of the sampling pipe through a clamping groove. The device has the beneficial effects that the structure is novel, and the operation is convenient; as the base, the cover plate, the sampling pipe and the electromagnetic valve are arranged, a large number of sampling specimens can be accommodated; water areas with different depths can be sampled, research data are enriched, the accuracy of research results is guaranteed, a sampling specimen can be protected by arranging a protection box, a floating plate, a box door and an electric push rod, meanwhile, the sampling specimen can be prevented from being polluted by external soil, air and the like in the lifting process, and the safety of the sampling specimen is guaranteed.

The invention discloses a peripheral blood TCR marker of a lung cancer, and a detection kit and an application thereof. The marker comprises at least one of proteins as shown in a sequence SpecSeq1-100. Based on a high-throughput sequencing method, only a small amount of peripheral blood needs to be adopted, RNA needs to be extracted, an immune map library is established by processing a sample, acharacteristic TCR sequence in the lung cancer peripheral blood is determined firstly through high-throughput sequencing and TCR data analysis, and then a test result of a sample to be tested is compared with the characteristic TCR sequence so that whether a lung cancer exists or not is determined. The marker, the kit and the application can be used to simultaneously compare a huge number of lungcancer specific TCR sequences, have higher specificity and accuracy compared with single detection of one or more markers, and improve diagnosis efficiency.

The invention discloses an apparatus for measuring dust content of flue gas and a method thereof. The apparatus comprises a dust collecting device, a sampling device and a suction device; an outlet end of the dust collecting device is connected with an inlet end of the sampling device, and the outlet end of the sampling device is connected with the inlet end of the suction device; in the method, the flue gas passes the dust collecting device under the condition of constant speed suction, the dust in the flue gas is filtered by filter paper, and the dust content of the flue gas is calculated by weighing the weight of the filter paper before filtering the dust and the weight of the filter paper after filtering the dust and putting the weights into a formula. By the device for measuring the dust content of the flue gas, the dust content of the flue gas can be accurately analyzed. The device has the advantages of novel design, simple structure and easy implementation, and is very applicable to large scale popularization and use. The method for measuring the dust content of the flue gas has the advantages of safe and reliable sampling, simple operation, accurate and reliable analysis result and fast detection.

The invention discloses a peripheral blood transcription gene marker for breast cancer detection. The peripheral blood transcription gene marker comprises blood cell characteristic gene sequences of breast cancers shown in SEQ ID NO.1-SEQ ID NO.10. The invention also discloses application of the gene marker in preparation of breast cancer detection products. According to the peripheral blood transcription gene marker, early screening detection is carried out on the breast cancers through combinatory analysis on the expression conditions of transcription gene markers related to the breast cancers in peripheral blood. The peripheral blood transcription gene marker has the advantages of high sensitivity and strong specificity, the peripheral blood which is most easily collected clinically is taken as a detection sample, sampling is safe, simple and convenient, the peripheral blood transcription gene marker is very suitable for early screening and auxiliary diagnosis of the breast cancers of a large-scale population, and the application prospect is wide.

The invention discloses a probe of an FDT (Formation Dynamic Test) module type formation tester of a formatting dynamic monitoring system, belonging to the technology of oil-gas exploration. The probe comprises a probe piston, a packer, a probe body, a connecting plate, a connecting screw, a filter sleeve, a filter piston, a probe plug, an elastic block, a positioning sleeve, a baffle ring, a piston body, a pressure-limiting valve, a guide block, a fixing screw and a fluid conveying pipe. According to the probe disclosed by the invention, the area of a formation which is packed by the packer is enlarged, a plurality of diversion trenches are added beside a sampling port of the packer, consequently, liquid sampling is facilitated, and the FDT module type formation tester can be more effectively and safely used in an unconsolidated formation, an oil sheet and a low permeable formation; the FDT module type formation tester is more reliable in sealing, more convenient in positioning and faster in sampling; and the FDT module type formation tester is more convenient and reliable to use, wider in application range and more outstanding in effect.

The invention provides a closed sampling device which comprises: a sampling needle comprising a liquid feeding needle, a gas discharging needle, a liquid inlet communicated with the liquid feeding needle, and a gas outlet communicated with the gas discharging needle; a protective cover component comprising a protective cover, and a support ring connected with the protective cover; and a sampling bottle component comprising a sampling bottle, a bottle cap, and an elastic gasket located between the sampling bottle and the bottle cap. The closed sampling device of the invention is equipped with the sampling needle; sampling is carried out by puncturing the elastic gasket sealed at the opening of the sampling bottle by the liquid feeding needle and the gas discharging needle on the sampling needle; the sampling and gas discharging processes are completed in closed environment; during sampling, the requirements of zero leakage and zero discharge are reached; and the sampling device of the invention is convenient, rapid and safe in sampling, and causes no environment pollution.

The invention discloses a peripheral blood TCR marker for colorectal cancer as well as a detection kit and application of the peripheral blood TCR marker. The marker comprises at least one of proteinsas shown in a sequence SpecSeq1-100. The sequence is based on a high-throughput sequencing method; only a small amount of peripheral blood needs to be collected; RNA is extracted, a sample is treatedto establish an immune map library, high-throughput sequencing and TCR data analysis are carried out, firstly, a characteristic TCR sequence in colorectal cancer peripheral blood is determined, and then a test result of a sample to be tested is compared with the characteristic TCR sequence so as to determine whether a patient suffers from colorectal cancer or not. The kit can simultaneously compare a huge number of colorectal cancer specific TCR sequences, has higher specificity and accuracy compared with single detection of one or more markers, and improves the diagnosis efficiency.

The invention discloses a peripheral blood TCR marker for ovarian cancer as well as a detection kit and application of the marker. The marker comprises at least one of proteins shown as SEQ ID NO.1-100 in sequence. The method is based on a high-throughput sequencing method and includes steps of: drawing only a small amount of peripheral blood, extracting RNA (Ribonucleic Acid), treating a sample to establish an immune map library, carrying out high-throughput sequencing and TCR data analysis, determining a characteristic TCR sequence in peripheral blood of the ovarian cancer, and then comparing a test result of the sample to be tested with the characteristic TCR sequence so as to determine whether the ovarian cancer exists or not. The marker can simultaneously compare a huge number of ovarian cancer specific TCR sequences, has higher specificity and accuracy compared with single detection of one or more markers, and improves the diagnosis efficiency.

The invention discloses a peripheral blood TCR marker for novel coronavirus infection as well as a detection kit and application thereof. The peripheral blood TCR marker comprises at least one of proteins shown in a sequence SpecSeq1-100. Based on a high-throughput sequencing method, only a small amount of peripheral blood needs to be adopted, RNA needs to be extracted, an immune map library is established by processing a sample, then through high-throughput sequencing and TCR data analysis, a characteristic TCR sequence in the peripheral blood infected by the novel coronavirus is determined firstly, then a test result of the sample to be tested is compared with the characteristic TCR sequence, and therefore, whether the patient suffers from the novel coronavirus infection or not is determined. According to the peripheral blood TCR marker for the novel coronavirus infection and the detection kit and application thereof, the huge number of the novel coronavirus infection specific TCR sequences can be simultaneously compared, the specificity and the accuracy are high compared to the single detection of one or more markers, and the diagnosis efficiency is improved.

The invention relates to an unlimited depth self-propelled riverbed material sampling device. The sampling device comprises a working ship, a composite steel cable, a support frame and a sampler, thesampler comprises an information bin, a power bin, a sample bin, a sand discharging cone and a leakage-proof part. The sampler and the support frame sink together through the composite steel cable onthe working ship, and the water depth is not limited; the sampler can be vertically suspended and automatically and integrally pushed downwards when being close to the bed surface, and the sampling depth is not limited. The counter weight is pulled by the chain to roll along the cylinder wall in a three-point simple supporting mode and ascend and descend in the air, and it is guaranteed that resistance is small; the total length of the thin-wall pipe orifice and the sampler is relatively short so that the energy of push-down driving is relatively small; the extrusion deformation of the sand sample in the short pipe type sample bin is very small, and the bin cover is independently opened and closed to realize high fidelity of sampling; the leakage-proof part ensures that the sand sample does not slip and leak; and a water pressure gauge in the sampler detects the depth of the sampling point away from the bed surface. Sampling of the device is not limited by depth, and the riverbed material sample can be safely, reliably and accurately collected in a high-fidelity mode.

The invention discloses a peripheral blood TCR (T cell receptor) marker for breast cancer and a detection kit and an application thereof. The marker comprises at least one of proteins shown as SEQ IDNO.1-100 in sequence. The method is based on a high-throughput sequencing method, only a small amount of peripheral blood needs to be collected, the method comprises the following steps of extractingRNA (Ribonucleic Acid), treating a sample to establish an immune map database, carrying out high-throughput sequencing and TCR data analysis, firstly determining a characteristic TCR sequence in peripheral blood of breast cancer, and then comparing a test result of a sample to be tested with the characteristic TCR sequence so as to determine whether the patient suffers from breast cancer or not. The marker can simultaneously compare a huge number of breast cancer specific TCR sequences, has higher specificity and accuracy compared with single detection of one or more markers, and improves diagnosis efficiency.

The invention discloses a peripheral blood TCR marker of acute B lymphocytic leukemia as well as a detection kit and application of the peripheral blood TCR marker. The peripheral blood TCR marker comprises at least one of proteins of which the sequences are as shown in SEQ ID NO.1-100. On the basis of a high-throughput sequencing method, only a small amount of peripheral blood needs to be adopted, RNA is extracted, an immune map library is established by treating a sample, then through high-throughput sequencing and TCR data analysis, firstly, a characteristic TCR sequence in the peripheral blood of the acute B lymphocytic leukemia is determined, and then a test result of a to-be-tested sample is compared with the characteristic TCR sequence, therefore, whether a patient suffers from acute B lymphocytic leukemia is determined. According to the peripheral blood TCR marker, a huge number of acute B lymphocytic leukemia specific TCR sequences can be compared at the same time, and the peripheral blood TCR marker has higher specificity, higher accuracy and improved diagnosis efficiency compared with single detection of one or more markers.

Synchronization is attained between a source clock domain and a target clock domain of arbitrary frequency ratios and each of which periodically has edges nominally aligned to edges of a reference clock signal, marked by the assertion of a periodic sync signal. The periodic sync signal, synchronous with the source clock, is used to output to an unload pointer counter in the target clock domain the deassertion of a reset signal prior to the nominal alignment of the source clock and the target clock for sampling on the nominally aligned target clock edge. The deassertion of the reset signal is output to a load pointer in the source clock domain coincident with the nominally-aligned edges of the source clock and the target clock. Both loading and unloading start based on the reset deassertion being sampled on the nominally aligned edges in the appropriate clock domain.

The invention relates to a passive isolation sampling circuit for the output direct current of a three-phase rectifier bridge. The output direct current of the conventional three-phase rectifier bridge is sampled by a current divider, so that interference over the transmission of a signal line is easily produced. A three-phase rectifier bridge output circuit rectifies three-phase alternating current into direct current, and the direct current is transmitted to a load through a direct current filter reactor. The heavy three-phase alternating current is converted into alternating current signal voltage with low amplitude through an incoming current transformer and a current conversion resistor, and the three-phase alternating current signal voltage is converted into direct current signal voltage through a signal rectifier bridge by adopting a Y connection method. The amplitude of the direct current signal voltage completely corresponds to the direct current according to a certain sampling proportional relationship, the amplitude of the direct current is correspondingly displayed by a parallel direct current voltmeter, and the output of the signal rectifier bridge is connected in parallel with a capacitor for filtration. The Y connection method is adopted for independent sampling, so that an isolation property is high; and the sampling circuit is not required to be connected with a major loop, so that high voltage influence is avoided, loss and heat are reduced, and sampling safety and convenience are ensured.

The invention relates to equipment and a method capable of safely acquiring aquatic organism samples and an acquisition method, wherein a central processing unit (CPU) is connected with a fractional-frequency comparison module IC3, a 16-digit high-precision data acquisition card DAQ, a digital display module IC4 and a voltage level comparison module IC6, respectively; the fractional-frequency comparison module IC3 is connected with light-emitting diodes LED4 and LED5 and a loudspeaker Y; the fractional-frequency comparison module IC3 is connected with a photocoupling circuit module IC5; the photocoupling circuit module IC5 is connected with a magnetic field conversion unit module MT, an inductor L1 and a monitoring switch K8; the monitoring switch K8 is connected to an underwater output load resistor Z1. During acquisition, a carrying manner of the equipment, the types and the number of the aquatic organism samples to be acquired are determined, and a net gear is prepared; in addition, the range of the output electric field intensity is determined; an output electrode is put in water; the equipment is turned on to generate a pulse electromagnetic field in the water area surrounding the electrode; when the aquatic organism samples move to the position of the prepared net gear, the aquatic organism samples are gained. The equipment provided by the invention is light in weight and convenient to carry; the aquatic organism samples are complete and safe to use; the acquisition rate is high and effective group data can be obtained.