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Molecular marker and kit for detecting COVID-19 susceptibility, and application of molecular marker

A technology for COVID-19 and molecular markers, applied in molecular markers, kits and application fields for detecting susceptibility to COVID-19, capable of solving the problem of no genetic variation of susceptibility to COVID-19, no detection kits and detection methods, etc. problems, to achieve the effect of high accuracy, fast detection process and simple operation

Pending Publication Date: 2021-10-22
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, there are no reports of genetic variations associated with susceptibility to COVID-19, and there are no related detection kits and detection methods

Method used

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  • Molecular marker and kit for detecting COVID-19 susceptibility, and application of molecular marker
  • Molecular marker and kit for detecting COVID-19 susceptibility, and application of molecular marker
  • Molecular marker and kit for detecting COVID-19 susceptibility, and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Detection of FOXP4 gene mutation and its correlation analysis with COVID-19 infection;

[0029] 1.1 Screening subjects:

[0030] Select 200 cases of confirmed patients with new coronary pneumonia without complications, and select 200 cases of age- and gender-matched healthy control groups. At least 1.5 mL of peripheral blood was collected from the subjects and stored in a -80°C refrigerator for future use.

[0031] 1.2 Extract sample DNA:

[0032] The DNA in the peripheral blood of the subjects in the above step 1.1 was extracted; wherein, in this embodiment, the blood sample DNA was extracted using the QIAGEN DNA Purification Kit, Cat. No.: 69504.

[0033] The specific operation process is as follows:

[0034] Take 100uL blood sample, add 20uL proteinase K, 100uL PBS shake and mix;

[0035] Add 200uL Buffer AL, shake and mix well, then bathe in water at 56°C for 10min;

[0036] Add 200uL of absolute ethanol, shake and mix to obtain a mixture;

[0037...

Embodiment 2

[0060] Apply the COVID-19 susceptibility detection kit to judge the susceptibility of the subject to COVID-19;

[0061] According to the relevant results of Example 1, it was found that the FOXP4 gene is closely related to the susceptibility to COVID-19, and gene-specific primers can be designed based on the mutation in this result, thereby making a detection kit for detecting the blood of the tested patient DNA template.

[0062] The above detection kit (taking 100 person-times as an example) contains the reagents shown in Table 3 below.

[0063] Table 3 Kit content list

[0064]

[0065] Wherein, the sequence list of the above primers is shown in Table 4 below;

[0066] Table 4 Primer sequence list

[0067]

[0068] At the same time, the PCR reaction mixture contains the usual components of the PCR reaction: Buffer, Taq enzyme, dNTP, Mg 2+ and double distilled water.

[0069] In practical application, the kit includes a packaging box, a sponge liner, a test tube w...

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Abstract

The invention discloses a molecular marker and a kit for detecting COVID-19 susceptibility, and application of the molecular marker. A method disclosed in the invention comprises the following steps: (1) amplifying an FOXP4 gene of a sample by adopting specific primers as shown in SEQ ID No: 2 and SEQ ID No: 3 to obtain an amplification product; and (2) carrying out sequence determination on the amplification product obtained in the step (1), determining that the FOXP4 gene is a mutant FOXP4 gene when the 1007 site of the FOXP4 gene sequence has an SNP site and deoxynucleotide is changed from C to A, and determining that the FOXP4 gene is a normal gene when the FOXP4 gene is still C. By detecting whether an in-vitro sample contains the single nucleotide polymorphism of the FOXP4 gene or not, early diagnosis and screening of COVID-19 high-risk groups and susceptible groups and effective prevention and control of COVID-19 can be realized.

Description

technical field [0001] The present invention relates to gene single nucleotide polymorphism (single nucleotide polymorphism, SNP) and its correlation research with COVID-19, belongs to the field of molecular biology and medical technology, specifically relates to a method for detecting COVID-19 Molecular markers, kits and applications of susceptibility. Background technique [0002] Estimates from data modeling studies and sero-infection studies estimate the actual number of infections to be much larger, suggesting that most infected individuals may have mild symptoms or be asymptomatic. Patients with COVID-19 exhibit a wide range of clinical symptoms: up to 5% of confirmed cases develop severe pneumonia including acute respiratory distress syndrome (ARDS). Although current studies have found that factors such as advanced age, male sex, hypertension, diabetes, obesity, and cardiovascular disease are closely related to the onset of COVID-19, there are still a large number of...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113
Inventor 马丁吴鹏丁文成邬堂春王超龙刘晨
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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