Short serial repeating sequence universal probe and design method and application thereof

A technology of short tandem repeats and probes, applied in biochemical equipment and methods, recombinant DNA technology, DNA/RNA fragments, etc., can solve the problems of complex cost, difficult to reach the detection range, high cost, etc., and achieve shortened design length, high Effect of annealing temperature and detection specificity and high accuracy

Pending Publication Date: 2019-10-18
领航医学科技(深圳)有限公司
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Problems solved by technology

Digital PCR technology is used for non-invasive prenatal genetic testing. The copy number of a single site is very low, and it is difficult to achieve the best detection range. Therefore, it is necessary to take the total copy number for analysis after multiple site detection
The current digital PCR probe design needs to consider the base sequence, which is complicated and expensive

Method used

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  • Short serial repeating sequence universal probe and design method and application thereof
  • Short serial repeating sequence universal probe and design method and application thereof
  • Short serial repeating sequence universal probe and design method and application thereof

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Embodiment Construction

[0045]The technical solutions of the present invention will be described in detail below in conjunction with the drawings and embodiments, but the present invention is not limited to the scope of the embodiments.

[0046] For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions. The reagents and raw materials used in the present invention are all commercially available.

[0047] Main technical scheme of the present invention is as follows:

[0048] First, the tandem repeat sequence is used as the core sequence to design the probes. The numbers of the universal probe 1, probe 2, probe 3 and probe 4 are: FAM-P, VIC-P, CY5-P1 and CY5-P2 , the specific nucleotide sequence is shown in Table 1.

[0049] Table 1

[0050] Numbering sequence GC% Number of bases FAM-P FAM-tctctctcacacacac-MGB (SEQ NO: 1) 50％ 16 VIC-P ...

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Abstract

The invention relates to the field of in-vitro diagnosis, and more specifically relates to a universal probe for simultaneously detecting multiple sites of a genome, and a primer design method and anapplication kit thereof. The universal probe adopts a short tandem repeat sequence as a skeleton sequence, and solves the problems that the number of the probes in a reaction system in which the copynumber concentration is amplified is too large, and non-specific amplification is easily formed and the synthesis cost is high, and the concentration of the chromosome copy number can be proportionally amplified by designing multiple pairs of the primers on both sides of the universal probe position of the single copy gene fragment of the chromosome to-be-tested, which is especially suitable for the detection of certain very low concentration samples (such as whether the non-invasive detection of chromosome copy number generates variation or not).

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a short tandem repeat sequence universal probe and its design method and application. Background technique [0002] In the late 1980s, James of the Marshfield Medical Research Foundation and Wis of Oregon Health and Science University and others isolated a satellite DNA with shorter repeating units than small satellite DNA, called microsatellite DNA (Microsatellite DNA), Short tandem repeats (Short Tandem Repeats, STRs) or simple sequence repeats (Simple Sequence Repeats, SSRs), the length of each repeat unit is between 1 and 6 bp. STR conforms to the Mendelian inheritance pattern, is co-dominantly expressed, and is widely distributed in the genomes of eukaryotes. According to the repeated motif, it can be divided into three types: single type (such as (AC)n), compound type (such as (AC)m (AG)n) and discontinuous type (such as (AC)m T(AG)n). According to the number of repeated...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2525/151C12Q2531/113C12Q2561/101C12Q2563/107C12Q2563/159
Inventor 朱海涛李旭新夏江
Owner 领航医学科技(深圳)有限公司
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