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Blood vancomycin drug concentration monitoring kit and detection method thereof

A technology of vancomycin and reagent kits, which is applied in the field of medical testing, can solve the problems of high technical requirements for experimenters, inaccurate qualitative and quantitative analysis, and long analysis time, so as to reduce errors and personnel operation errors, and the detection process is simple and fast. The effect of cost reduction

Inactive Publication Date: 2019-10-22
苏州艾迪迈医疗科技有限公司
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Problems solved by technology

Previous methods have problems such as complicated pre-treatment, inaccurate qualitative and quantitative results, high cost, long analysis time, or high technical requirements for experimenters.

Method used

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  • Blood vancomycin drug concentration monitoring kit and detection method thereof
  • Blood vancomycin drug concentration monitoring kit and detection method thereof
  • Blood vancomycin drug concentration monitoring kit and detection method thereof

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Embodiment Construction

[0035] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with illustrations and specific embodiments.

[0036] 1. Preparation of Vancomycin Therapeutic Drug Monitoring Kit:

[0037] Standard curve reagent: where vancomycin is prepared into a series of concentrations with animal serum containing 2% stabilizer and 2% plasticizer, respectively 0.6mg / L, 2.5mg / L, 5.0mg / L, 8.0mg / L, 13.0mg / L, 18.0mg / L, 1.0ml per bottle. The above components were freeze-dried and stored at the bottom of the assay bottle.

[0038] Quality control reagent: among them, vancomycin was formulated with animal serum containing 2% stabilizer and 2% plasticizer to three concentrations of high, medium and low, respectively 1.0mg / L, 8.0mg / L, and 16.0mg / L. The bottle is filled with 1.0ml. The above components were freeze-dried and stored at the bottom of the assay...

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Abstract

The invention relates to the field of medical detection and in particular to a blood vancomycin therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquid chromatography analytical technology, more specifically to a method for accurately and quantitatively detecting vancomycin drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. The therapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has various methodological indicators that satisfy the requirement for monitoring the blood vancomycin therapeutic drugs, and is easy to generalize.

Description

technical field [0001] The present invention relates to the field of medical testing, in particular to a kit for monitoring vancomycin therapeutic drug in blood developed based on multi-dimensional online solid-phase extraction liquid chromatography analysis technology, more specifically, it adopts online solid-phase extraction and multi-dimensional liquid phase Chromatographic analysis technology is a method for accurate and quantitative monitoring of vancomycin drug concentration in blood. Background technique [0002] Vancomycin belongs to glycopeptide antibiotics, and its hydrochloride is used clinically. Vancomycin has a strong bactericidal effect on Gram-positive cocci and some Gram-positive bacilli (such as diphtheria, Clostridium). The mechanism of antibacterial activity of vancomycin is mainly to inhibit the synthesis of bacterial cell walls. Vancomycin can also change the permeability of bacterial cell membranes and affect the synthesis of RNA. This compound mecha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/16G01N30/34G01N30/86
CPCG01N30/06G01N30/16G01N30/34G01N30/8631
Inventor 杨晓洪石功名何伶秀管燕
Owner 苏州艾迪迈医疗科技有限公司
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