Building method of mouse model with myocardium transfected with lentivirus

Abstract

The invention relates to a building method of a mouse model with myocardium transfected with lentivirus. By the method, myocardium administration uniformity and stability can be increased. During myocardium injection in the method, a syringe is used to inject medicine or lentivirus into the ventricular wall muscle of the left ventricle of the heart in a way that the trace syringe is used, needle injection depth is the paraboloid length of the needle, and three-point injection is performed. By the method, needle injection depth can be strictly controlled, the effect of the medicine or lentivirus can be reinforced, and experiment uniformity and stability can be increase.

Description

technical field [0001] The invention relates to a method for constructing a mouse model transfected with a lentivirus in the myocardium. The model can ensure the uniformity and stability of myocardial administration. Background technique [0002] With the improvement of social level and the aggravation of population aging, the morbidity and mortality of cardiovascular diseases are rising rapidly. Although the prevention and treatment of cardiovascular diseases in my country has achieved preliminary results, it still faces severe challenges. At the same time, since the average annual growth rate of hospitalization expenses for cardiovascular and cerebrovascular diseases is much higher than the growth rate of GDP, the economic burden brought by cardiovascular diseases is also increasing. Getting worse. In recent years, although gene therapy and stem cell therapy have provided the possibility to cure cardiovascular diseases, the most ideal way to study whether a certain gene pl...

AI Technical Summary

Problems solved by technology

Currently, most of the lentivirus injections used in cardiovascular disease research use tail vein injection. However, due to the large amount of virus injected into the tail vein, it is easy to fail and cause infection, and the transfection efficiency cannot be confirmed at the first time. Therefore, the myocardial injection technology will slowly The efficient introduction of foreign substances such as viruses into the heart tissue and their stable expression will play a great role in the study of cardiovascular diseases

[0004] Mice, as a classic model animal, can be well used in the study of heart diseases. However, when performing myocardial injection experiments, due to the small size of the mouse heart and the thickness of the ventricle wall is only 1.0~1.3mm, it is difficult to perform precise injection. , A little carelessness will inject into the heart cavity, resulting in the loss of the injected substance with the blood circulation, unable to exert its efficiency, and in severe cases, the heart will bleed and the mouse will die

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