Method for preparing outer membrane protein H and A genes of fowl cholera and fused DNA vaccine

Abstract

The invention discloses a method for preparing outer membrane protein H and A genes of fowl cholera and a fused DNA vaccine, which comprises the following nine items of: obtaining the outer membrane protein H and A genes of the fowl cholera by utilizing a polymerase chain reaction method; performing enzyme digestion, recycling and purification on the outer membrane protein H gene of the fowl cholera; performing enzyme digestion, recycling and purification on a eukaryotic expression vector pcDNA3.1(+); connecting and converting after purification; extracting pcM plasmid solution; performing enzyme digestion, recycling and purification on the pcM plasmid solution; performing enzyme digestion, recycling and purification on the outer membrane protein A gene of the fowl cholera; connecting theA gene with the pcM plasmid solution and converting; and preparing the fused DNA vaccine from the H and A genes. Animal experiments prove that the fused DNA vaccine prepared by the method can effectively improve the capacity of immunized chicken of resisting the attack of avian pasteurella multocida, has the protection ratio of 75 percent and can effectively prevent the fowl cholera caused by theavian pasteurella multocida.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines, in particular to a method for preparing fowl cholera outer membrane protein H and A genes and a fusion DNA vaccine. [0002] The title of the above invention is limited by the number of words in the title, the full name of fowl cholera outer membrane protein H and A gene should be: fowl cholera outer membrane protein H gene and fowl cholera outer membrane protein A gene, so the preparation of fowl cholera outer membrane protein H and fowl cholera outer membrane protein The method for A gene and fusion DNA vaccine should be: the method for preparing fowl cholera outer membrane protein H gene and fowl cholera outer membrane protein A gene and fusion DNA vaccine. Background technique [0003] Fowl cholera is a contagious poultry infectious disease caused by Pasteurella multocida. It is distributed around the world. It is also a common multiple infectious disease in my country. Almost all pou...

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Problems solved by technology

[0009] So far, there has been no relevant report on the method of obtaining fusion DNA vaccine by preparing fowl cholera outer membrane protein H gene and fowl cholera outer membrane protein A gene