Method for increasing growth speed of crucian carps based on transgenes

Abstract

The invention belongs to the technical field of genetic engineering, and discloses a method for increasing growth speed of crucian carps based on transgenes. The method for increasing growth speed ofcrucian carps based on transgenes comprises the following steps of performing fst gene cloning of the crucian carps, performing extraction to obtain total RNA of muscular tissue of the crucian carps,performing reverse transcription to obtain cDNA, and using the cDNA as a formwork; performing PCR amplification to obtain a sequence; constructing a transgene carrier, and through BamHI and Xmal enzyme cutting, inserting a target gene fragment in the sequence so as to form the transgene carrier, wherein the transgene carrier contains main elements of Tol2-mylz2-fst-rabbit globin-Tol2, a mylz2 promoter enables a target gene to be specifically expressed in the muscular tissue, and rabbit globin can increase the stability of the mRNA of the target gene; and performing microinjection so as to obtain the transgenic crucian carps.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a method for increasing the growth speed of crucian carp based on transgene. Background technique [0002] At present, the closest existing technology: at present, the most in-depth development of fast-growing transgenic fish is the growth hormone-transformed yellow river carp developed by the team of academician Zhu Zuoyan, and then growth hormone-transformed trout, salmon, crucian carp, crucian carp, etc. have been produced one after another. . However, growth hormone as a target gene has the following disadvantages. First, people have a certain degree of fear when talking about hormones, which affects the promotion of transgenic fish, even though growth hormone is a protein hormone. Secondly, in some fishes, the overexpression of the growth hormone gene does not significantly promote the growth of fish. For example, the overexpression of the salmon gro...

AI Technical Summary

Problems solved by technology

However, compared with the four major family carp, the biggest disadvantage of crucian carp is its slow growth rate, which seriously restricts the further development of crucian carp aquaculture industry.

[0004] In summary, the existing problems in the prior art are: the target gene used in the development of transgenic fast-growing fish is mostly the growth hormone gene, however, overexpressing the growth hormone gene in some fish cannot obtain the fast-growing transgenic fish species, Therefore, there is an urgent need to find other target genes that can promote fish growth, and it was found that the growth rate of the transgenic crucian carp was significantly accelerated

However, compared with the four major family carp, the biggest disadvantage of crucian carp is its slow growth rate, which seriously restricts the further development of crucian carp aquaculture industry.

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