Novel method for detecting creatinine in urine on basis of SERS (surface enhanced Raman spectroscopy)

A technology of surface-enhanced Raman and detection methods, which is applied in Raman scattering, measurement devices, preparation of test samples, etc., can solve problems affecting creatinine Raman response, eliminate competition effects, improve Raman response, and detect The effect of high sensitivity

Inactive Publication Date: 2019-11-15
CAS HEFEI INST OF TECH INNOVATION
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Problems solved by technology

However, SERS has a competing effect, and the presence of a large amount of urea in urine will affect the Raman response of creatinine

Method used

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  • Novel method for detecting creatinine in urine on basis of SERS (surface enhanced Raman spectroscopy)
  • Novel method for detecting creatinine in urine on basis of SERS (surface enhanced Raman spectroscopy)
  • Novel method for detecting creatinine in urine on basis of SERS (surface enhanced Raman spectroscopy)

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Embodiment Construction

[0009] Combine below Figure 1 to Figure 3 , the present invention is described in further detail.

[0010] refer to figure 1 , a novel method for detecting creatinine in urine based on surface-enhanced Raman spectroscopy, comprising the following steps: (A) taking normal urine and urine to be detected, removing urea in the urine; Add creatinine powder to normal urine to make creatinine samples with standard concentrations; (C) SERS substrate synthesis; (D) collect creatinine samples and remove the urine to be detected after the SERS spectrum reacts on the SERS substrate, and perform spectral analysis on the spectral data Process to obtain the creatinine concentration in the urine to be detected. By removing urea in urine, the strong adsorption of -NH2 group of urea on SERS-enhanced gold / silver particles can prevent the adsorption of creatinine molecules in urine, thereby eliminating the presence of a large amount of urea in urine SERS detection. The competitive effect of t...

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Abstract

The invention particularly relates to a novel method for detecting creatinine in urine on basis of SERS (surface enhanced Raman spectroscopy). The method comprises the steps as follows: (A) taking normal urine and to-be-detected urine and removing urea in the urine; (B) adding creatinine powder to the normal urine with urea removed, and producing a standard concentration creatinine sample; (C) synthesizing an SERS substrate; (D) acquiring SERS after the creatinine and the to-be-detected urine with urea removed react on the SERS substrate, and processing spectroscopic data to obtain concentration of creatinine in the to-be-detected urine. By removal of urea in the urine, the condition that strong adsorption of -NH2 groups of the urea to enhanced substrate gold / silver particles hinders adsorption of creatinine molecules in the urine can be avoided, the competitive effect caused by a large quantity of urea in SERS detection of the urine is eliminated, the Raman response of creatinine in the urine is improved, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to the technical field of creatinine detection in urine, in particular to a novel creatinine detection method in urine based on surface-enhanced Raman spectroscopy. Background technique [0002] A urine test is a medical testing method. Including routine urine analysis, detection of formed components in urine (such as urine red blood cells, white blood cells, etc.), quantitative determination of protein components, urine enzyme determination, etc. Urine examination is of great value in clinical diagnosis, judgment of curative effect and prognosis. Creatinine is an important indicator of kidney function. The normal range of urine creatinine in humans is: 8.4-13.25mmol / 24h urine or 40-130mg / dL (ie 400-1300mg / L); an increase in creatinine content usually means substantial damage to the kidneys rear. Traditional creatinine detection methods such as chromatography, mass spectrometry, enzyme-linked immunoassay, etc., are easily affec...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/34
CPCG01N1/34G01N21/658
Inventor 徐青山郑守国邱梦情翁士状刘瑜凡王海燕朱恭钦孙熊伟
Owner CAS HEFEI INST OF TECH INNOVATION
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