Method for prolonging shelf life of arma chinensis eggs

A technology of shelf life and beetles, applied in the field of insects, can solve problems such as unfavorable large-scale application and short shelf life, and achieve the effects of delayed development and long shelf life

Inactive Publication Date: 2019-11-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no diapause phenomenon of stinkbug, and there are problems such as short shelf life of stinkbug eggs, which is not conducive to large-scale application

Method used

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  • Method for prolonging shelf life of arma chinensis eggs
  • Method for prolonging shelf life of arma chinensis eggs
  • Method for prolonging shelf life of arma chinensis eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Take the artificial climate box as an example for the storage place of stinkbug eggs, clean it 24 hours before use, and spray and disinfect it twice with 75% (volume percentage) ethanol to ensure the cleanliness of the artificial climate box; put the stinkbug eggs to be stored into Put the clean petri dish covered with filter paper in the artificial climate box, lower the temperature to the specified temperature T1 (10°C) and T2 (15°C) at a rate of 2°C every 30min, at 65±5%RH, 16 :8(L:D) conditions to store the stinkbug eggs; take out 30 stinkbug eggs every two days, hatch at normal temperature, record the hatching rate, and set 3 replicates. The incubation period of stinkbug eggs stored at room temperature was used as a control. figure 1 .

[0053] figure 1 It is the result of detecting the hatching rate of stink bugs at different storage temperatures and storage periods under the condition of no shading treatment. Depend on figure 1 It can be seen that under the con...

Embodiment 2

[0055] Take the artificial climate box as an example for the storage place of stinkbug eggs, clean it 24 hours before use, and spray and disinfect it twice with 75% (volume percentage) ethanol to ensure the cleanliness of the artificial climate box; put the stinkbug eggs to be stored into In a clean Petri dish covered with filter paper, put the Petri dish in a light-tight box to avoid light and then store it in an artificial climate box, and lower the temperature to the specified temperature T1 ( 10°C) and T2 (15°C), store the stinkbug eggs under the condition of 65±5% RH; take out 30 stinkbug eggs every two days, hatch them at normal temperature, record the hatching rate, and set 3 repetitions. The incubation period of stinkbug eggs stored at room temperature was used as a control. figure 2 .

[0056] figure 2 It is the result of detecting the hatching rate of stinkbug under different storage temperature and storage period under the condition of shading treatment. Depend...

Embodiment 3

[0058] Collect the newly produced stinkbug eggs into a 50ml centrifuge tube, add 75% (volume percentage) ethanol to submerge the stinkbug eggs for 10 minutes (vibration 3 times, each time 15s), and use a pipette to suck up the liquid in the centrifuge tube; Add 4% (volume percent) formaldehyde solution to cover the stinkbug eggs for 5 minutes (shaking once, time 15s), use a pipette gun to suck up the liquid in the centrifuge tube; add 75% (volume percent) ethanol to cover the stinkbug eggs Stinkbug eggs were treated for 4 minutes (shaking once, time 15s), and the liquid in the centrifuge tube was aspirated with a pipette gun; the egg surface reagent was rinsed with sterilized deionized water, and then placed on sterilized filter paper to blot the surface moisture. EG group. Those who did the above treatment with sterilized deionized water were the PG group, and those who had not undergone any treatment were used as the control group, that is, the CG group. Under the condition...

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PUM

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Abstract

The invention relates to a method for prolonging the shelf life of arma chinensis eggs. The method comprises the step (1) and / or the step (2), wherein (1) parent arma chinensis eggs are treated by using a solution with a disinfecting effect and water, hatching, feeding, mating and oviposition are conducted, and breeding is performed under a shading condition; (2) the arma chinensis eggs are subjected to gradient cooling and storage under the shading condition. The method has the advantage that the storage life of the arma chinensis eggs is long, and the method has important significance on production of arma chinensis and preservation of germplasm resources.

Description

technical field [0001] The invention belongs to the technical field of insects, and in particular relates to a method for prolonging the shelf life of stinkbug eggs. Background technique [0002] The long-term use of chemical pesticides seriously threatens the food and ecological security of our country, and also greatly affects the national health; the stink bug belongs to the Hemiptera stinkbug family, and is a natural enemy insect with a wide range of predation. It can prey on leaf beetles and cotton bollworms. The larvae or adults of more than 40 kinds of agricultural and forestry pests, such as silver-leaved moths, slug moths, scarabs, and aphids. [0003] Biological control centered on the use of natural enemy insects can effectively control the number of pests and reduce the use of pesticides. Doing a good job in the indoor propagation of natural enemy insects is the primary task when it is applied to pest control. There is no diapause phenomenon of stinkbug, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01N1/02
CPCA01K67/033A01N1/02A01N1/0284
Inventor 刘晨曦廖平陈红印张礼生王孟卿毛建军李玉艳殷焱芳
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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