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PVY-CP serving as target of drug for screening and treating potato Y virus and application thereof

A technology of PVY-CP and potato, which is applied in the field of gene function and application, can solve the problems of unclear mechanism of action and difficulty of special drugs

Active Publication Date: 2019-11-26
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the current basic research on potato virus Y disease has progressed, and there are a few drugs on the market that can effectively control this disease, but the specific mechanism of action is still unclear, making it extremely difficult to develop specific drugs against this pathogen

Method used

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  • PVY-CP serving as target of drug for screening and treating potato Y virus and application thereof
  • PVY-CP serving as target of drug for screening and treating potato Y virus and application thereof
  • PVY-CP serving as target of drug for screening and treating potato Y virus and application thereof

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Embodiment Construction

[0019] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0020] 1. Experimental materials

[0021] (1) Plasmid and expression strain

[0022] PVY was purchased from Wuhan Institute of Virology, Chinese Academy of Sciences;

[0023] BL21(DE3) RIL strain was purchased from Takara Company;

[0024] Nicotiana benthamiana and Nicotiana benthamiana K326 can be cultivated in the artificial climate greenhouse of the Agricultural College of Guizhou University.

[0025] (2) Tested proteins and anti-plant virus small molecules

[0026] PVY-CP protein was expressed and purified; benzothiadiazole and morpholinidine hydrochloride were provided by Guangxi Tianyuan; amino-oligosaccharins were purchased from Guangxi Beihai Guofa Marine Biological Pesticide Co., Ltd.; ribavirin was purchased from Hangzhou Minsheng Pharmaceutical Co., Ltd.

[0027] (3) The preparation method of the main buffer solution

[0028] LB solid medium: 10g...

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Abstract

The invention belongs to the field of gene function and application and relates to PVY-CP serving as a target of a drug for screening and treating potato Y virus and an application thereof. The invention provides the target of the drug for screening and treating the potato Y virus, wherein the target is potato Y virus coat protein, that is, PVY-CP. The invention also provides an application of thepotato Y virus coat protein, that is, the PVY-CP, serving as the target of the drug for screening and treating the potato Y virus. The invention further provides the drug for screening and treating the potato Y virus, wherein the drug takes the potato Y virus coat protein, that is, PVY-CP, as the target. For the action mechanism of the PVY-CP in the virus transmission process, the PVY-CP is usedas the target of potential antiviral agents for the first time; in vitro screening of drug active small molecules is conducted, and activity detection of small pharmaceutical molecules in live tobaccoplants is carried out; and a binding protein immunoblotting proves that the PVY-CP can serve as the target of the drug for screening and treating the potato Y virus.

Description

technical field [0001] The invention relates to a PVY-CP as a target for screening drugs for preventing and treating potato virus Y and its application, which belongs to the field of gene function and application. Background technique [0002] In recent years, plant virus diseases have brought huge losses to the quality and quantity of agricultural products in the process of agricultural production, among which the virus diseases caused by potato virus Y (Potato virus Y, or PVY) are particularly serious. The way PVY infects plants is systemic infection. Plants infected with PVY can show different symptoms in the field, which is closely related to PVY virus strains, crop varieties, and local climate conditions and other factors. Generally speaking, in the early stage of the disease, the leaves will have bright veins, and then the color between the veins of the leaves will gradually become lighter, and finally form mottled systems and mosaic symptoms. In some plants, when th...

Claims

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Application Information

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IPC IPC(8): G01N33/68A01N43/653A01N43/16A01N47/44A01N43/82A01P1/00
CPCG01N33/68A01N43/653A01N43/16A01N47/44A01N43/82Y02A50/30
Inventor 李向阳谢鑫刘涛
Owner GUIZHOU UNIV
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