Application of Interleukin 4 Receptor in Preparation of Drugs for Prevention and Treatment of Enterovirus 71 Infection

Abstract

The invention relates to the technical field of biomedicine, in particular to a new target for resisting enterovirus 71 infection and its application. In the present invention, human colon cancer cells (Caco-2) are used as target cells, and RNA interference technology is used to down-regulate the expression of target cell membrane receptors to find host factors that can effectively inhibit EV71 from infecting human colon cancer cells. The purpose of blocking EV71 infection. The present invention finds through experiments that interleukin 4 receptor (IL4R) plays an important role in the infection of Caco-2 cells by EV71, and down-regulates the expression of IL4R, which can obviously inhibit the infection of EV71. The invention provides the application of IL4R in the preparation of drugs for the prevention or treatment of enterovirus 71 infection, which provides clinical prevention and treatment of diseases such as hand, foot and mouth disease, meningitis, brainstem encephalitis or polio caused by EV71 infection. new targets and treatment options.

Description

technical field

[0001] The invention relates to the technical field of biomedicine, in particular, a new target and application for resisting enterovirus 71 infection. Background technique

[0002] Enterovirus 71 (enterovirus 71, EV71) belongs to the picornaviridae enterovirus and belongs to human enterovirus A, and is one of the main pathogens causing hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children can develop into severe patients with central nervous system (central nervous system , CNS) lesions, including aseptic meningitis, brainstem encephalitis, encephalomyelitis, and neurogenic pulmonary edema, which seriously threaten the life and heal...

Examples

Embodiment 1

[0062] 1 Design and synthesis of specific siRNA sequences for each transport-related membrane protein.

[0063] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis on AXIIR, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each ge...

technical field

[0001] The invention relates to the technical field of biomedicine, in particular, a new target and application for resisting enterovirus 71 infection. Background technique

[0002] Enterovirus 71 (enterovirus 71, EV71) belongs to the picornaviridae enterovirus and belongs to human enterovirus A, and is one of the main pathogens causing hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children can develop into severe patients with central nervous system (central nervous system , CNS) lesions, including aseptic meningitis, brainstem encephalitis, encephalomyelitis, and neurogenic pulmonary edema, which seriously threaten the life and heal...

Examples

Embodiment 1

[0062] 1 Design and synthesis of specific siRNA sequences for each transport-related membrane protein.

[0063] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis on AXIIR, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each ge...

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