Method for making guinea pig MCAO (middle cerebral artery occlusion) model
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A technology for arterial occlusion and production method, applied in the direction of animal husbandry, etc., can solve the problems of inability to achieve reperfusion effect, ligation, etc., and achieve the effect of ensuring reperfusion effect, high success rate, and reasonable and accurate method of wire insertion
Inactive Publication Date: 2019-12-27
昆明医科大学第二附属医院
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[0004] The purpose of the present invention is to provide a method for making a guinea pig arterial occlusion MCAO model, to solve the traditional common carotid artery embolization method proposed in the above-ment...
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Embodiment 1
[0029] A kind of preparation method of guinea pig arterial occlusion MCAO model, comprises the following steps:
[0030] S1: The anesthetic is prepared now, the guinea pig is weighed, and a certain amount of anesthetic is injected intraperitoneally according to the weight of the guinea pig to anesthetize the guinea pig;
[0031] S2: Place the guinea pig on a temperature-controlled blanket and fix it on the operating table to ensure that the body of the guinea pig is straight and without tension. Use a hair clipper to shave off the hair on the neck of the guinea pig, and use Aner Iodine for local disinfection;
[0032] S3: Cut the skin along the midline of the guinea pig’s neck, cut the anterior cervical fascia to expose the right sternocleidomastoid muscle, and then separate the anterior neck muscle group and sternocleidomastoid muscle, stretch it with a thin silk thread, Separate the muscle tissue until the intact CCA of the common carotid artery is observed;
[0033] S4: Se...
Embodiment 2
[0046] A kind of preparation method of guinea pig arterial occlusion MCAO model, comprises the following steps:
[0047] S1: The anesthetic is prepared now, the guinea pig is weighed, and a certain amount of anesthetic is injected intraperitoneally according to the weight of the guinea pig to anesthetize the guinea pig;
[0048] S2: Place the guinea pig on a temperature-controlled blanket and fix it on the operating table to ensure that the body of the guinea pig is straight and without tension. Use a hair clipper to shave off the hair on the neck of the guinea pig, and use Aner Iodine for local disinfection;
[0049] S3: Cut the skin along the midline of the guinea pig’s neck, cut the anterior cervical fascia to expose the right sternocleidomastoid muscle, and then separate the anterior neck muscle group and sternocleidomastoid muscle, stretch it with a thin silk thread, Separate the muscle tissue until the intact CCA of the common carotid artery is observed;
[0050] S4: Se...
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Abstract
The invention discloses a method for making a guinea pig MCAO (middle cerebral artery occlusion) model, which comprises the steps of preparing an anesthetic, weighing a guinea pig, and injecting a certain amount of the anesthetic into the abdominal cavity according to the weight of the guinea pig, so that the guinea pig is anesthetized; placing the guinea pig on a temperature control blanket, fixing the guinea pig on an operating table with the body of the guinea pig straight and free of tension, scraping the neck hair of the guinea pig with a hair clipper, and locally sterilizing with entoiodine; cutting the skin along the paracervical median of the guinea pig to open the anterior cervical fascia and expose the sternocleidomastoid muscle on the right side, then separating the anterior cervical muscle group and sternocleidomastoid muscle, and drawing with a fine silk thread to separate the muscle tissues until a complete CCA (common carotid artery) is observed. Compared with the traditional common carotid artery plug-in method, the method has the advantages that blood is directly supplied from the common carotid artery through the internal carotid artery after reperfusion, the common carotid artery is directly ligated after reperfusion, the reperfusion blood supply can only be circulated through lateral branches, the reperfusion effect similar to the clinical effect can be achieved, and the success rate of model preparation is high.
Description
technical field [0001] The invention relates to the technical field of animal models, in particular to a method for making an MCAO model of guinea pig arterial occlusion. Background technique [0002] The brain is the most sensitive organ of the human body to hypoxia. Brain tissue ischemia will cause damage to local brain tissue and its function. The degree of damage is related to the length of ischemia time and the amount of residual blood flow. Reversible damage, and prolonged complete ischemia or severe ischemia can cause infarction, histological changes The most obvious histological changes in the brain are cerebral edema and brain cell necrosis, the middle cerebral artery is the most common involvement of ischemic stroke Blood vessels, the traditional common carotid artery embolization method, directly ligates the common carotid artery after reperfusion, and the blood supply of reperfusion can only be through the collateral circulation, which cannot achieve the reperfus...
Claims
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