Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of ccfm16 in the preparation of bacterial agents, food or medicine for alleviating autism and adsorbing polychlorinated biphenyls

A technology of CCFM16 and polychlorinated biphenyls, which is applied in the application fields of bacterial agents, food or medicine, can solve the problems of neuron function damage, etc., and achieve the effect of alleviating social barriers, increasing normal stretching rate, and improving cognitive ability

Active Publication Date: 2022-04-15
JIANGNAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous activity and proliferation of glial cells (astrocytes and microglia) with morphological changes, the release of various pro-inflammatory mediators (cytokines and chemokines) to coordinate immune responses and regulate neuronal function and connectivity, while this response can be neuroprotective (i.e., help repair damaged tissue), chronic dysregulation of CNS cytokines can lead to disruption of neuronal function through their roles in cognition and synaptic regulation, These cytokines include IL-6 and TNF-α, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ccfm16 in the preparation of bacterial agents, food or medicine for alleviating autism and adsorbing polychlorinated biphenyls
  • Application of ccfm16 in the preparation of bacterial agents, food or medicine for alleviating autism and adsorbing polychlorinated biphenyls
  • Application of ccfm16 in the preparation of bacterial agents, food or medicine for alleviating autism and adsorbing polychlorinated biphenyls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Bifidobacterium bifidum CCFM16 has good tolerance to simulated gastrointestinal fluid

[0045] The cryopreserved Bifidobacterium bifidum CCFM16 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and subcultured in mMRS medium for 2~ After 3 times, take 1 mL of the culture solution of Bifidobacterium bifidum CCFM16, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0h, 0.5h, 1h, and 2h, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0046] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. Take...

Embodiment 2

[0047] Embodiment 2: Bifidobacterium bifidum CCFM16 has no toxic and side effects to wistar rats

[0048] Bifidobacterium bifidum CCFM16 bacteria were resuspended in 10% skim milk to prepare a bacterial suspension with a concentration of 3.0×10 8 CFU / mL. Six male wistar autistic rats with a body weight of about 200 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0049] These test results showed that feeding Bifidobacterium bifidum CCFM16 at a concentration of 3.0×10 8 CFU / mL had no significant impact on rats, no significant changes in body weight, and no death. The appearance of the rats showed no obvious pathological symptoms.

Embodiment 3

[0050] Example 3: Bifidobacterium bifidum CCFM16 can significantly improve the autonomous exploration ability of autistic rats

[0051] (1) Breeding of young mice with autism

[0052] Healthy male and female wistar rats were bred and adapted to the environment for 1 week. They were caged together at 5:00 p.m. at a ratio of 2:1, and the vaginal plug was observed at 8:00 the next morning. If no vaginal plug was observed, a vaginal smear was performed. On the first day of pregnancy, the female mice were housed in separate cages after conception, and randomly divided into model group and control group. On the 12.5th day after pregnancy, intraperitoneal injection of sodium valproate (VPA) 600mg / kg (normal saline made into 250mg / mL solution) was given as the model group; Weaning.

[0053]

[0054] (2) Experimental grouping of autistic rats

[0055] Divided into control group, model group and intervention group.

[0056]

[0057] (3) Open field test of autistic rats

[005...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of CCFM16 in the preparation of bacterial agents, food or medicine for relieving autism and absorbing polychlorinated biphenyls. Bifidobacterium bifidum CCFM16 can improve autonomous behavior and exploration in unfamiliar environments caused by autism Behavior, relieve social barriers caused by autism, significantly improve stereotyped behaviors caused by autism, improve autism's ability to recognize new objects, improve the decline in limb coordination caused by nerve damage, and increase the normal body extension rate , Relieve muscle rigidity and posture and gait disorders caused by Parkinson's disease, and effectively adsorb PCBs.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to the application of CCFM16 in the preparation of bacterial agents, food or medicine for relieving autism and adsorbing polychlorinated biphenyls. Background technique [0002] Autism (Autism), also known as autism, has been recognized as a generalized neurodevelopmental disorder that frequently occurs in infants and young children, mainly manifested in social interaction impairment, communication (verbal and non-verbal) function impairment, and repetitive stereotyped behaviors. , often accompanied by a variety of clinical symptoms such as mental retardation, epilepsy or hyperactivity, usually before the age of 3. According to the latest data released by the US Centers for Disease Control and Prevention (CDC), the prevalence of ASD in 2018 was 1:59, a 15% increase from 1:68 in 2016, and the prevalence rate of men was 4 to 5 times that of women. . [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123A23L11/00A23L19/20A23L29/00A61K35/745A61P25/00C12R1/01A23L11/50
CPCA23C9/1234A23L19/20A23L29/065A61K35/745A61P25/00C12N1/20A23V2002/00A23L11/50A23V2200/322A23V2200/30
Inventor 王刚陈卫孔庆敏赵建新张灏
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com