Digital PCR (dPCR) system and dPCR droplet forming method

A droplet and digital technology, applied in chemical instruments and methods, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of small flux, slow droplet formation speed, low utilization rate of PCR oil, etc., to reduce oil The effect of reducing the amount of phase consumption and consumption

Active Publication Date: 2020-02-21
SHANGHAI IND U TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a digital PCR system and a method for forming digital PCR droplets, which are used to solve the problems of slow droplet formation, small flux, complicated operation, and PCR in the prior art. The problem of low oil utilization

Method used

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  • Digital PCR (dPCR) system and dPCR droplet forming method
  • Digital PCR (dPCR) system and dPCR droplet forming method
  • Digital PCR (dPCR) system and dPCR droplet forming method

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Embodiment 1

[0106] The present invention provides a digital PCR system, please refer to Figure 1a to Figure 1g ,in Figure 1a Shown as a schematic diagram of the three-dimensional structure of the digital PCR system, Figure 1b is shown as a top view of the digital PCR system, Figure 1c is shown as a bottom view of the digital PCR system, Figure 1d , Figure 1e , Figure 1f and Figure 1g They are respectively shown as side views of the digital PCR system in four directions. figure 2 It is shown as a schematic diagram of an exploded structure of the digital PCR system. In this embodiment, the digital PCR system includes at least one droplet forming component 1 and a droplet orifice component 3, and the droplet orifice component 3 is connected to the Droplet forming assembly 1 below.

[0107] Specifically, the droplet forming assembly 1 includes at least one droplet collection tank 2 . Figure 1a Shown in is the case where the number of the droplet forming assembly 1 is one, and ...

Embodiment 2

[0139] The present invention also provides a digital PCR liquid droplet forming method, comprising the following steps: using a vaporization component to vaporize the digital PCR solution and rapidly pushing it into the droplet forming oil to form the digital PCR liquid droplet.

[0140] As an example, a thermal bubble technique is used for high-speed digital PCR droplet formation, and the vaporization component includes a heating component to vaporize the liquid layer of the digital PCR solution by heating it.

[0141] Specifically, the formation speed of the digital PCR droplets is controlled by controlling the heating time, heating frequency and heating interval time of the heating component. Using the digital PCR droplet forming method of the present invention can achieve a digital PCR droplet formation speed greater than 1000 / second.

[0142] As an example, the digital PCR droplet forming method includes the following steps:

[0143] S1: Inject digital PCR solution into ...

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Abstract

The invention provides a digital PCR (dPCR) system and a dPCR droplet forming method. The dPCR system comprises at least one droplet forming assembly and a droplet spray hole assembly, wherein each droplet forming assembly comprises at least one droplet collection groove, the droplet spray hole assembly is connected to the lower side of the at least one droplet forming assembly and comprises multiple droplet spray holes, the droplet spray holes communicate with the at last one droplet collection groove, and vaporization components are arranged in the droplet spray holes and used for vaporizingdPCR solution liquid layers in the droplet spray holes and quickly pushing the vaporized liquid layers into droplet forming oil in the at least one droplet collection groove to form dPCR droplets. The dPCR droplet forming method forms dPCR droplets at high speed by a thermal bubble technology, can realize the droplet forming speed larger than 1,000 per second and has the efficient dPCR oil utilization rate.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to the field of disease detection, and relates to a digital PCR system and a digital PCR droplet forming method. Background technique [0002] It has been 20 years since the polymerase chain reaction (PCR) was proposed. During this period, PCR has developed into a key technology and a routine technology in the field of molecular biology, which has greatly promoted the development of various fields of life science. Especially in the late 1990s, the real-time fluorescence quantitative PCR (real time PCR, qPCR) technology and related products launched by the American ABI company developed PCR from in vitro synthesis and qualitative / semi-quantitative detection technology into a highly sensitive and highly specific method. and precise quantitative genetic analysis techniques. [0003] Although after more than ten years of rapid development, qPCR technology has been used in the diagnosis of al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/00C12Q1/6851
CPCB01L3/502784B01L7/525C12Q1/6851B01L2300/0838B01L2400/0487C12Q2563/159C12Q2565/629C12Q2531/113C12M1/38C12M1/36C12M1/00B01L2200/10B01L2300/048B01L2400/0442B01L3/502715
Inventor 吴炫烨关一民
Owner SHANGHAI IND U TECH RES INST
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