A kind of method for preparing scallop muscle single cell suspension

Abstract

The invention relates to a method for preparing a single-cell suspension of scallop muscle, which belongs to the technical field of marine invertebrate cell biology. The specific steps of the method are as follows: cutting the adductor muscle tissue into 1-2mm 3 Add enzyme mixture for complete digestion, transfer to centrifuge tube after digestion, collect cell sediment by centrifugation twice, add pre-cooled CMFSS solution to cell sediment, resuspend cells by pipetting, filter, add buffer again CMFSS, pipetting and resuspension, obtains scallop muscle single-cell suspension; the method of the present invention can realize low-cost, fast and high-efficiency acquisition of Ezo scallop muscle single-cell suspension, and ensure that the dissociated cells maintain good vitality, and can be applied to The dissociation, isolation and purification of other marine shellfish cells and the establishment of cell lines provide important reference materials for the study of single cells, gene functions and genetic breeding of Ezo scallop.

Description

technical field [0001] The invention belongs to the technical field of marine invertebrate cell biology and relates to a method for preparing scallop muscle single-cell suspension. Background technique [0002] Patinopecten yessoensis belongs to cold-water shellfish, and its origin is distributed in sea areas such as Japan, the Russian Far East and North Korea. The scallop species with the highest economic value. The adductor muscle of Ezo scallop is hypertrophic, tender, and rich in nutrition. It is favored by consumers at home and abroad, and it is a high-end and best-selling aquatic product in the aquatic product market. Although my country is a big country in scallop farming, it is not yet a strong country in scallop farming. There are problems such as relatively lagging genetic improvement research, relatively weak genetic basic research, and lack of improved species, which seriously affect and restrict the sustainable development of my country's scallop farming indust...

AI Technical Summary

Problems solved by technology

However, different tissues have their own unique physiological characteristics, which is the main reason why shellfish cell culture has become a worldwide problem

The adductor muscle tissue is the main edible part of scallops. Existing experimental techniques are still unable to obtain single-cell suspensions of scallop muscle with good cell viability, which restricts the cell culture, establishment of cell lines and functional gene verification of scallop adult muscle tissue. research work

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