A product that can be used to prevent and/or treat Salmonella infections
A Salmonella, product technology, applied in the field of biomedicine, can solve problems such as increased antibiotic content, intestinal dysfunction, and unsatisfactory results
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Embodiment 1
[0058] Embodiment 1: The influence of eugenol on the growth of Lactobacillus plantarum ZS2058 and Salmonella
[0059] The bacterial suspension of Lactobacillus plantarum ZS2058 and the bacterial suspension of Salmonella were respectively analyzed by MRS - The culture medium was diluted to a concentration of 2×10 6 CFU / mL, to obtain the dilution of Lactobacillus plantarum ZS2058 and the dilution of Salmonella; in the dilution of Lactobacillus plantarum ZS2058 and the dilution of Salmonella, add concentrations of 0mmol / L, 0.0125mmol / L, 0.025mmol / L Eugenol in L, 0.05mmol / L, 0.1mmol / L, 0.2mmol / L, 0.4mmol / L, 0.8mmol / L, 1.6mmol / L, 3.2mmol / L, 6.4mmol / L, 12.8mmol / L, Cultivate statically at 37°C. During the culture period, measure the OD of the dilution with a microplate reader every 1 hour for the first 8 hours 600 , Measure the OD of the diluent every 2 hours from 8 to 12 hours 600 To detect the growth curves of Lactobacillus plantarum ZS2058 and Salmonella under the influence of ...
Embodiment 2
[0063] Example 2: Effects of eugenol and / or Lactobacillus plantarum ZS2058 on Salmonella adhesion, invasion ability and virulence gene expression
[0064] With RMPI-1640 medium containing 5% (v / v) CO 2 Cultivate HT-29 cells in a 37°C incubator until the cell density of HT-29 cells reaches 80%, transfer to a 6-well plate to obtain a well plate full of HT-29 cells; the well plate full of HT-29 cells Divided into four groups, namely Ctrl group, eugenol group (E), Lactobacillus plantarum ZS2058 group (Z) and Lactobacillus plantarum ZS2058 combined eugenol group (ZE), wherein, add 1 × 10 9 For the Salmonella of CFU / well, add 0.2mmol / L eugenol and 1×10 9 For the Salmonella of CFU / well, add 1×10 9 CFU / well of Lactobacillus plantarum ZS2058 and 1×10 9 For the Salmonella of CFU / well, add 0.2mmol / L Eugenol, 1×10 9 CFU / well of Lactobacillus plantarum ZS2058 and 1×10 9 CFU / well of Salmonella, four groups of orifice plates were cultured in a 37°C constant temperature incubator for 4 ...
Embodiment 3
[0068] Example 3: Effects of eugenol and / or Lactobacillus plantarum ZS2058 on the survival rate of Salmonella-infected mice
[0069] Take 30 7-week-old female C57BL / 6 mice, and feed them adaptively for 1 week under the conditions of room temperature 20-26°C, humidity 40-70%, 12h / 12h day and night, and free access to food and water. Divided into 5 groups, 6 rats in each group, the 5 groups were blank control group (C), model group (M), eugenol group (E), Lactobacillus plantarum ZS2058 group (Z) and Lactobacillus plantarum ZS2058 combined with eugenol group (ZE).
[0070] Dilute the Salmonella suspension with PBS buffer to a concentration of 1 × 10 7 CFU / mL to obtain the Salmonella dilution; dilute Lactobacillus plantarum ZS2058 with PBS buffer to a concentration of 1×10 7 CFU / mL to obtain a dilution of Lactobacillus plantarum ZS2058; dilute eugenol with PBS buffer to a concentration of 300 mg / mL to obtain a dilution of eugenol.
[0071] The experiment lasted for 25 days: mic...
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