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Platycodon grandiflorum fermentation method

A technology of platycodon grandiflorum and platycodon grandiflora powder, applied in the direction of fermentation, microbial-based methods, biochemical equipment and methods, etc.

Pending Publication Date: 2020-03-17
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no efficient method to increase the content of active ingredients in Platycodon grandiflorum by fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 10g platycodon root dry powder, 50g water, the addition of glucose is 5%, is mixed with fermentation medium; Inoculate lactic acid bacteria seed liquid 3.5mL (viable bacteria number 1×10 6 CFU / mL), angel yeast 0.175g; ferment at 25°C for 1 day.

[0042] Ultrasonic extraction was performed with 70% ethanol at a power of 200W for 1 hour, and the extract was rotary evaporated and freeze-dried into powder.

[0043] The contents of total saponins, total polysaccharides, total flavonoids and total polyphenols in the freeze-dried powder were determined by ultraviolet spectrophotometry: 5.44%, 8.37%, 1.33%, 2.89%.

Embodiment 2

[0045] 10g Platycodon grandiflorum dry powder, 40g water, the addition of glucose is 4%, is mixed with fermentation medium; Inoculate lactic acid bacteria seed liquid 3.5mL (viable bacteria number 1×10 6 CFU / mL), Angel yeast 0.175g, fermented at 25°C, and the fermentation time was 1d.

[0046] Ultrasonic extraction was performed with 70% ethanol at a power of 200W for 1 hour, and the extract was rotary evaporated and freeze-dried into powder.

[0047] The contents of total saponins, total polysaccharides, total flavonoids and total polyphenols in the freeze-dried powder were determined to be 4.93%, 9.11%, 1.21%, and 2.68%.

Embodiment 3

[0049] 10g Platycodon grandiflora dry powder, 60g water, the addition of glucose is 4%, is mixed with fermentation medium; Inoculate 3.5mL of lactic acid bacteria seed liquid (viable bacteria number 1×10 6 CFU / mL), Angel yeast 0.175g, fermented at 25°C for 2 days.

[0050] Ultrasonic extraction was performed with 70% ethanol at a power of 200W for 1 hour, and the extract was rotary evaporated and freeze-dried into powder.

[0051] The contents of total saponins, total polysaccharides, total flavonoids and total polyphenols in the freeze-dried powder were determined to be 4.53%, 9.11%, 2.59%, and 3.11%.

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Abstract

The invention provides a platycodon grandiflorum fermentation method and belongs to the technical field of platycodon grandiflorum fermentation. The method comprises the following steps: 1) mixing platycodon grandiflorum powder, glucose and water to obtain a fermentation culture medium, and 2) inoculating the fermentation medium with lactic acid bacteria and saccharomycetes and carrying out fermentation to obtain a fermentation product, wherein the mass ratio of the platycodon grandiflorum powder to the water is 1: (4-6), the mass percentage of the glucose in the fermentation culture medium is3%-8%, the ratio of the inoculation volume of the lactic acid bacteria to the mass of the fermentation medium is (2-10) mL: (50-70) g, the ratio of the inoculation mass of the saccharomycetes to themass of the fermentation culture medium is (0.1-0.5): (50-70) and the fermentation time is 0.5 to 3 days. According to the method disclosed by the invention, the content of active ingredients including total saponins, total polysaccharides, total flavonoids and total polyphenols in platycodon grandiflorum can be increased in a targeted manner.

Description

technical field [0001] The invention belongs to the technical field of bellflower fermentation, in particular to a method for bellflower fermentation. Background technique [0002] Campanulaceae is a plant of the Campanulaceae family, mainly distributed in Henan, Hebei, Liaoning and Jilin in my country. Among them, wild platycodon grandiflorum grown in Changbai Mountain is the top grade. Platycodon grandiflorum is mild in nature, bitter and pungent in taste, and is a traditional wild Chinese herbal medicine in my country that is also used as medicine, food and ornamental. [0003] Judging from the current research status of platycodon grandiflorum, most of the research is still on the analysis of platycodon grandiflorum components and other pharmacological effects. There are relatively few reports on whether platycodon grandiflorum has hypoglycemic effect and the active ingredients related to hypoglycemic effect. If the research on the biological activity of Platycodon gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/00C12P7/02C12P33/00C12N1/20C12N1/18C12R1/01C12R1/865
CPCC12N1/18C12N1/20C12P7/02C12P19/00C12P33/00C12P39/00
Inventor 崔承弼齐欣于婷金莉英姜欣吴松权
Owner YANBIAN UNIV
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