Method for determining verbascoside in blood-nourishing and brain-refreshing water extract

A technology of extracting glucoside and water extract, which is applied in the field of detection and analysis, and can solve the problems of inability to achieve the detection purpose, long analysis time, and lack of content determination of glucoside

Active Publication Date: 2020-04-07
TIANJIN TASLY MORDEN TCM RESOURCES
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Problems solved by technology

The content of verbascoside in Yangxue Xuenao water extract is only about one-fifth of the test product in the control literature, and it is a mixed extract. The detection method of Rehmannia glutinosa in Part 1 of "Chinese Pharmacopoeia" 2015 edition was used. Mutual interference, long analysis time, low efficiency, unable to achieve the purpose of detection, for this reason, a better separation and purification method is needed to detect the content of verbascoside
[0007]Currently, there is no test for the content of verbascoside in the brain water extract of nourishing serum

Method used

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  • Method for determining verbascoside in blood-nourishing and brain-refreshing water extract
  • Method for determining verbascoside in blood-nourishing and brain-refreshing water extract
  • Method for determining verbascoside in blood-nourishing and brain-refreshing water extract

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Embodiment 1

[0169] Embodiment 1: content detection method

[0170] Chromatographic conditions: ACQUITY UPLC HSS T3 C18 (2.1*100mm) chromatographic column, with 0.2% formic acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, gradient elution, detection wavelength: 334nm, gradient elution Table 7:

[0171] Table 15: Gradient Elution Table

[0172]

[0173] Preparation of reference solution:

[0174] Weigh an appropriate amount of verbascoside reference substance, add methanol to prepare a solution containing 0.004 mg of verbascoside per milliliter, and obtain it.

[0175] Preparation of the test solution:

[0176] Accurately weigh about 0.25 g of Yangxue Xuenao water extract, add about 10 ml of 25% methanol, dissolve it by ultrasonic, shake well, and put it on a ProElut C18-U or Sep-Pak Vac C18 solid phase extraction column. Wash with 5 ml of 25% methanol and discard. Elute with 5mL of 40% methanol solution, collect the eluate into a 5ml volumetric flask, add 40%...

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Abstract

The invention relates to a method for determining verbascoside in blood nourishing and brain-refreshing water extract. The method comprises the following steps: 1) reference substance preparation method: weighing a verbascoside reference substance, and adding methanol to prepare a standard reference solution; 2) a preparation method of a test solution: weighing the blood nourishing and brain refreshing water extract, adding methanol to dissolve, loading on a solid phase extraction column, eluting with a methanol solution, collecting eluent, and fixing the volume with methanol; and 3) a determination method: respectively taking the reference substance solution and the test solution, injecting into an ultra-high performance liquid chromatograph, recording a chromatogram, calculating the verbascoside content of the test solution by using an external standard method according to the peak area, and converting to obtain the verbascoside content in the blood nourishing and brain refreshing water extract.

Description

technical field [0001] The invention belongs to detection and analysis technology, and in particular relates to a method for measuring verbascoside in nourishing serum brain water extract. Background technique [0002] Yangxueqingnao Granules and Yangxuexuenao Pills are Chinese patent medicines developed by Tasly Group, which are recorded in pages 1281-1282 and 1280-1281 of the 2015 edition of the Chinese Pharmacopoeia, respectively. During the preparation process of Yangxuexuenao Granules and Yangxuexuenao Pills, it is necessary to extract Chinese medicinal materials such as rehmannia glutinosa, Uncaria, Millae Spatholobus, Prunella vulgaris, mother of pearl, and Asarum, and prepare them into medicinal extracts, which are then used The extract is prepared into Yangxuexuenao Granules and Yangxuexuenao Pills from the active substance of the medicine. The preparation method of the medicinal extract extract is: Rehmannia glutinosa, Uncaria, Millae Spatholobus, Prunella vulgaris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 牛涛陈红徐波张学敏
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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