Procypris merus sperm cryopreservation method

Abstract

The invention discloses a procypris merus sperm cryopreservation method. The method comprises four steps of seminal fluid collection, seminal fluid dilution, sperm cryopreservation and sperm unfreezing, procypris merus seminal fluid is collected by adopting an artificial abdominal extrusion method, then the seminal fluid is mixed with a diluent, the mixture is mixed with antifreezing liquid, and sperm preservation liquid is obtained, and the diluent is any one of D-15 diluent, Hank's liquid and Ringer's Solution for fishes, wherein the antifreeze agent is any one of methanol, dimethyl sulfoxide and glycerol; the sperm preservation liquid is injected into a cryopreservation tube, and the cryopreservation tube immersed into liquid nitrogen for long-term preservation; when the sperm preservation liquid is taken, the cryopreservation tube filled with the sperm preservation liquid is taken out of the liquid nitrogen and then immediately placed in a water bath kettle at the temperature of 27DEG C or 37 DEG C or 47 DEG C to be unfrozen, and then obtained procypris merus sperms are used for artificial insemination. The method can preserve the procypris merus sperms for a long time, and the survival rate of the thawed and recovered sperms is high.

Description

technical field

[0001] The invention belongs to the technical field of biological low-temperature cryopreservation, and in particular relates to a method for cryopreservation of spermatozoa of the carp carp. Background technique

[0002] Hehua carp is a small warm-water fish of Cyprinidae, also known as Hehua black carp, Hehua carp, Hehua carp and Wu carp. Located in Guilin, Guangxi, it is a native Chinese fish and is a cultured species with local characteristics. The meat of Hehua carp is delicate. Less thorns and more meat, soft bones and no fishy smell, delicious taste, high protein content, and the mixed carp in the rice field has a mixed diet, fast growth, strong reproductive ability, short breeding cycle, and has high nutritional value and economical value. value.

[0003] With the increase in the scale of Hehua carp breeding year by year, the demand for Hehua carp seedlings is gradually increasing, and the quality requirements for Hehua carp seedlings are also incr...