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Application of nitazoxanide and tizoxanide in preparation of drug for resisting porcine reproductive and respiratory syndrome viruses

A technology for respiratory syndrome and nitazoxanide, which is used in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem that the mechanism of resisting certain viral infections is not fully elucidated.

Active Publication Date: 2020-04-17
武汉世纪金辉农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism of anti-virus infection has not been fully elucidated, and the application of nitazoxanide and tizoxanide in anti-animal virus has not been reported yet.

Method used

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  • Application of nitazoxanide and tizoxanide in preparation of drug for resisting porcine reproductive and respiratory syndrome viruses
  • Application of nitazoxanide and tizoxanide in preparation of drug for resisting porcine reproductive and respiratory syndrome viruses
  • Application of nitazoxanide and tizoxanide in preparation of drug for resisting porcine reproductive and respiratory syndrome viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Nitazoxanide and Tizoxonide Reduce the CPE of Porcine Reproductive and Respiratory Syndrome Virus Infected Cells

[0030] Nitazoxanide or tizoxanide was used to act on MARC-145 cells infected with porcine reproductive and respiratory syndrome virus (PRRSV), and its resistance to porcine reproductive and respiratory syndrome was detected by detecting the cytopathic changes (CPE) of MARC-145 cells. Virus (PRRSV) activity.

[0031] 1. Experimental materials and methods

[0032] Preparation of drug solution: Accurately weigh nitazoxanide and tizoxanide respectively and dissolve them in DMSO to prepare nitazoxanide solution and tizoxanide mother solution, and store at -4°C.

[0033]Cells and virus strains: African green monkey kidney (MARC-145) cells, preserved by the laboratory of the unit, the growth medium is DMEM medium with 10% FBS (Gibco-BRL, Gaithersburg, MD, USA), and the maintenance medium is DMEM medium containing DMEM medium with 2% FBS.

[0034] The...

Embodiment 2

[0049] Embodiment 2: Nitazoxanide and tizoxanide reduce the viral nucleic acid load experiment of porcine reproductive and respiratory syndrome virus (PRRSV) infected cells

[0050] 1. Experimental method

[0051] Drug treatment: Dilute the PRRSV virus to a corresponding concentration and add it to a 6-well plate so that each well contains 100 TCID50 of the virus, and then put it at 37°C and 5% CO 2 Incubate in the incubator for 2 hours, discard the virus solution and wash with PBS. Then add the corresponding concentrations of nitazoxanide and tizoxanide (0.5, 1, 1.5, 3, 6 μmol / L) diluted with 2% cell maintenance solution, add to the corresponding wells, add 2mL to each well, and continue to cultivate for 24h , the cell control group only added 2% cell maintenance solution.

[0052] Extract RNA:

[0053] 1) Discard the culture medium, add 350 μL Buffer RLT to each well of the 6-well plate according to the RNA extraction instructions, blow and mix with a pipette gun to fully...

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Abstract

The invention discloses an application of nitazoxanide and tizoxanide in the preparation of a drug for resisting porcine reproductive and respiratory syndrome viruses, and an application of a pharmaceutical preparation in the preparation of a drug for resisting porcine reproductive and respiratory syndrome virus infection. It is found in the invention that nitazoxanide and / or tizoxanide and pharmaceutically acceptable salts thereof can effectively protect animal cells at a low drug concentration, and the drug concentration can reach a micromole concentration level. It is also found in the invention that nitazoxanide and / or tizoxanide and pharmaceutically acceptable salts thereof can protect cells from virus infection and reduce the amount of nucleic acid of viruses in cells.

Description

technical field [0001] The present invention relates to the field of animal anti-virus, more specifically, relates to the application of nitazoxanide and tizoxanide in the preparation of anti-porcine reproductive and respiratory syndrome virus drugs. Background technique [0002] Nitazoxanide (NTZ), the chemical name is o-[N-(5-nitrothiazol-2-yl)carbamoyl]phenol acetate (2-(acetyloxy)-N-(5-nitro- 2-thiazolyl)benzamide), a thiazole drug, was first synthesized in the 1970s. Nitazoxanide is a prodrug, which is quickly hydrolyzed in the body to its active metabolite tizoxanide (TIZ) after administration, but the biological effects of the two are similar, and both have anti-parasitic and other pharmacological effects. [0003] Since the 1970s, people's understanding and development of nitazoxanide and tizoxanide have gradually increased. First of all, in order to deal with the harm of various protozoan diseases to humans and animals, people invented nitazoxanide and tizoxanide ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/625A61P31/14
CPCA61K31/625A61P31/14A61K2300/00
Inventor 韦淑亚徐圣郑楠
Owner 武汉世纪金辉农业科技有限公司
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