Preparation method and application method of biological stimulin capable of increasing succession cropping obstacle resistance of fragaria ananassa

A biostimulant, continuous cropping barrier technology, applied in the directions of botanical equipment and methods, chemicals for biological control, applications, etc., can solve problems such as few strawberries, achieve low cost, improve resistance, and be easy to industrialize effect of implementation

CN111052997AActive Publication Date: 2020-04-24QINGDAO AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2020-04-24

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Abstract

The invention discloses a preparation method and application method of a biological stimulin capable of increasing succession cropping obstacle resistance of fragaria ananassa. The biological stimulinconsists of bacillus cereus (Bacillus cereus) MGW6, CGMCC No.18689, bacillus (Bacillus sp.) MGW9, CGMCC No.18690, melatonin and a carrier glucose. The biological stimulin provided by the invention can relieve succession cropping obstacles of the fragaria ananassa and increase the succession cropping obstacle resistance of the fragaria ananassa, has the efficacy of promoting growth of plants, repairing succession cropping soil and the like, is particularly low in cost and simple to operate, and is easy in industrialization implementation.
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Description

technical field

[0001] The invention relates to a preparation and application method of a biostimulant for improving the resistance to continuous cropping obstacles of strawberry, which can alleviate the obstacles of continuous cropping of strawberries, improve the resistance of continuous cropping obstacles of strawberries, and has the effects of plant growth promotion and continuous cropping soil restoration, especially low cost, The operation is simple and easy for industrial implementation. Background technique

[0002] strawberry( Fragaria ananassa Duch) belongs to the Rosaceae (Rosaceae) strawberry plant. It is a fruit with high nutritional and economic value. It is very popular among consumers because of its good color, aroma and taste. In recent years, the area of ​​strawberry cultivation in my country has increased rapidly, but continuous cropping cultivation is prone to continuous cropping obstacles, resulting in abnormal plant growth and development, aggravation...

Examples

preparation example Construction

[0035] (3) Preparation of biostimulants: The target biostimulants mainly include microbial agent components, chemical agent components and carrier components.

[0036] The microbial inoculum component is composed of excellent target bacterial strain Bacillus cereus MGW6 and bacterial strain Bacillus sp. MGW9; the chemical agent component is melatonin, purchased from sigma company; the carrier component is glucose.

[0037] The main steps for preparing the target biostimulant involved in the present invention are:

[0038] First, select a fermenter of corresponding specifications according to the demand, and sterilize the tank body with high pressure steam before use, at 121°C for 45 minutes, and then culture the bacteria.

[0039] Secondly, the Bacillus cereus (Bacillus cereus) MGW6 and Bacillus sp. MGW9 were respectively inoculated into the beef extract peptone liquid medium in different tanks, the stirring speed was 150r / min, the culture temperature was 28°C, and the ventil...