Mixed microbial agent for degrading sulfonamide antibiotics in sewage and preparation method and application thereof

A technology for degrading sewage and mixed bacterial agents, which is applied in the field of environmental microorganisms to achieve good results, high-efficiency degradation, and simple production processes

Active Publication Date: 2020-04-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with mixed microbial communities, the disadvantages

Method used

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  • Mixed microbial agent for degrading sulfonamide antibiotics in sewage and preparation method and application thereof
  • Mixed microbial agent for degrading sulfonamide antibiotics in sewage and preparation method and application thereof
  • Mixed microbial agent for degrading sulfonamide antibiotics in sewage and preparation method and application thereof

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specific Embodiment approach 1

[0023] Specific embodiment one: a kind of mixed bacterial agent of degrading sulfa antibiotics in the sewage of the present embodiment, it is mixed by Arthrobacter YL1 and Pseudomonas YL2;

[0024] The Arthrobacter YL1 is Paenarthrobacter ureafaciens YL1, which is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is 2019. On August 6, the deposit number was CGMCC No.18365;

[0025] The Pseudomonas YL2 is Korean Pseudomonas (Pseudomonas koreana) YL2, which is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The date is August 6, 2019, and the deposit number is CGMCC No.18366.

specific Embodiment approach 2

[0026] Embodiment 2: This embodiment is different from Embodiment 1 in that Arthrobacter YL1 and Pseudomonas YL2 are mixed in a volume ratio of 1: (2-3). Others are the same as in the first embodiment.

[0027] This embodiment also provides a preparation and optimization method of a mixed bacterial agent for degrading sulfonamide antibiotics in sewage, the preparation process of which is as follows:

[0028] (1), the preparation of antibiotic mother liquor: accurately weigh 5g sulfa antibiotics, make up to 100mL with dimethyl sulfoxide (DMSO);

[0029] (2), colony culture:

[0030] ①. Preparation of basal salt medium: The medium composition is: 0.5g (NH 4 ) 2 SO 4 , 1.5g KH 2 PO 4 , 3.5g K 2 HPO 4 , 0.15g MgSO 4 ·7H 2 O, 0.5g NaCl, 1mL trace element and 2mL stock solution, filled to 1000mL with distilled water; the composition of the trace element solution is: 50g EDTA, 5.5g CaCl 2 2H 2 O, 5.06g MnSO 4 4H 2 O, 5g FeSO 4 ·7H 2 O, 2.2gZnSO 4 ·7H 2 O, 1.61g CoCl...

specific Embodiment approach 3

[0047] Embodiment 3: This embodiment is an application of a mixed bacterial agent for degrading sulfonamide antibiotics in sewage, which is used for the treatment of sulfa antibiotics in sewage or the degradation of organic matter in sewage.

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Abstract

The invention discloses a mixed microbial agent for degrading sulfonamide antibiotics in sewage and a preparation method and application thereof, belonging to the technical field of environmental microorganisms. The mixed microbial agent for degrading sulfonamide antibiotics in sewage is formed by mixing arthrobacter sp. YL1 and pseudomonas sp. YL2 in a volume ratio of 1: (2-3). The mixed microbial agent is used for treating sulfonamide antibiotics in sewage or degrading organic matters in sewage. The arthrobacter sp. YL1 and the pseudomonas sp. YL2 are separated from each other in the invention can use sulfonamide antibiotics as a unique carbon source for growth and can realize rapid and efficient degradation of the sulfonamide antibiotics; and compared with a single strain, the double-strain interaction microbial agent system has higher removal efficiency, more thorough removal amount and a more stable community structure in the process of practical application, and is very criticalto biological treatment of sulfonamide antibiotics in water. The mixed microbial agent of the invention is applied to the field of antibiotic degradation.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a mixed bacterial agent for degrading sulfonamide antibiotics in sewage, a preparation method and application thereof. Background technique [0002] Sulfonamides (SAs) are a class of artificial synthetic antibacterial drugs, mainly used for the prevention and treatment of bacterial infectious diseases. The commonly used sulfa drugs currently used in medicine and veterinary medicine mainly include sulfadiazine (SDZ), sulfaguanidine (SG), sulfamethoxazole (SMX), sulfamethazine (SM1), and sulfamethazine. (SM2) etc. However, because sulfonamide antibiotics cannot be completely metabolized by passive objects, most of them are still excreted in animal feces and urine in the form of original drugs or metabolites, and finally enter the environment through various ways to cause pollution. [0003] Biological treatment has the characteristics of low cost and environm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/34C02F101/38C12R1/06C12R1/38
CPCC12N1/20C02F3/34C02F2101/34C02F2101/38C02F2101/40C12R2001/06C12N1/205C12R2001/38
Inventor 马放于澜郭海娟王樱凝
Owner HARBIN INST OF TECH
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