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Double-channel microbial sensor type BOD (Biochemical Oxygen Demand) rapid tester for emergency detection

A microbial sensor, emergency detection technology, applied in specific-purpose bioreactors/fermenters, enzymology/microbiology devices, methods of supporting/immobilizing microorganisms, etc. Problems such as detection operations to achieve the effect of improving detection efficiency and accuracy

Pending Publication Date: 2020-04-28
清池水环境治理科技发展(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] ⑴The existing sensor-type BOD rapid detector (hereinafter referred to as the detector) does not meet the BOD detection requirements of emergency situations
[0013] ⑵Microbiological sensor detection unit, ①Because the dissolved oxygen electrode, microbial film, and the cell body of the flow cell are assembled and joined by rotary extrusion, mutual friction will occur when the three contact each other, especially when the screw is tight, Causes greater friction between the contact surfaces, causing deformation or even damage to the microbial membrane and the oxygen-permeable membrane
Directly affect the normal use of the analyzer, reduce the working efficiency of the analyzer and increase the operating cost
②Because the microbial membrane is a consumable, it needs to be replaced at any time. In this way, when assembling and replacing the microbial membrane, it is necessary to invert the dissolved oxygen electrode and the cell body, and disconnect the gas and liquid pipelines connected to the flow cell, resulting in cumbersome operations and costly time and manpower
③In the existing flow tank, the microbial membrane is directly placed in the membrane tank of the tank body. Every time the operator needs to take the thin, soft and fragile microbial membrane directly, it brings difficulty to the operator, low work efficiency and easy to cause damage. Microbial film damage
[0014] (3) The heating structure with electric heating block heating and aluminum column heat transfer has a large temperature gradient, and the constant temperature effect is not stable enough, which is not conducive to maintaining the ambient temperature required for microbial growth and affecting microbial activity. In severe cases, it cannot accurately reflect the consumption of microbial aerobic reactions The amount of oxygen directly affects the accuracy of test results
In addition, with this structure, when the liquid in the flow cell overflows, it will directly flow to the electric heating block and the surrounding electrical appliances or components, causing corrosion damage and a potential safety hazard
[0015] ⑷Sampling, air intake unit, air intake pump, pump body and adjustment knobs of the liquid intake peristaltic pump are all placed inside the analyzer case, if adjustment is required, the case cover must be opened for manual adjustment, and there is no intuitive quantitative data, adjust not sure
This manual operation is time-consuming, laborious and cumbersome, which is not conducive to the needs of rapid determination and emergency treatment.
In addition, the liquid and gas in the air inlet and liquid inlet pipelines are mixed to obtain a mixed gas liquid that directly enters the flow cell from the outside without preheating, which affects the temperature stability of the microbial membrane.
[0016] (5) The electrical control unit includes an electrical control main board, a display, a printer, an electrical socket and a control switch, but the display is only a display with a single display function, and the functional operation is completed through a keyboard, which is slow and inconvenient; and currently only temperature control is performed , and it uses a temperature probe built in the microbial sensor detection unit to cooperate with the control circuit, and the stability and accuracy are low
[0017] ⑹In addition, for the open-air detection operation carried out outdoors, the existing BOD rapid detector lacks a safety protection structure, is easily disturbed by the harsh and complex natural environment outside, and cannot complete the normal detection operation
Does not meet the requirements of fast, accurate and safe emergency testing

Method used

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  • Double-channel microbial sensor type BOD (Biochemical Oxygen Demand) rapid tester for emergency detection
  • Double-channel microbial sensor type BOD (Biochemical Oxygen Demand) rapid tester for emergency detection
  • Double-channel microbial sensor type BOD (Biochemical Oxygen Demand) rapid tester for emergency detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Use this tester to test the unity of the two electrodes, and the two channels are turned on at the same time. Put the left and right liquid-taking tubes 50a, 50b into the standard liquid bottles 52a, 52b containing 5mg / L, 10mg / L, 25mg / L, 35mg / L, and 50mg / L in turn, and turn on the intake pump switches 67a, 67b And peristaltic pump switch 68a, 68b, observe the reading of gas flow meter 43a, 43b, and manually adjust to the appropriate size; observe the reading on the peristaltic pump speed display 62 and adjust it to the appropriate size by touch screen display 63. Operate the touch screen display 63 to the standard curve mode, operate according to the prompts given by the touch screen display, and obtain two accurate standard curves. Then put the two-way liquid-taking tubes into the sample solution bottle 53 with a BOD of 20 mg / L respectively, and switch to the test mode by operating the touch screen display. After 8 minutes, the measuring instrument obtains the BOD valu...

Embodiment 2

[0084] Use this analyzer to compare with the traditional five-day biochemical method, and test the sample solutions with BOD of 5mg / L, 10mg / L, 25mg / L, 35mg / L, and 50mg / L respectively. Both channels are turned on at the same time. First, turn on the two-way intake pump switches 67a, 67b and peristaltic pump switches 68a, 68b, observe the readings of the gas flowmeters 43a, 43b, and manually adjust them to a suitable size; 63 adjust it to the appropriate size. Put the left and right liquid-taking tubes 50a, 50b of the tester into the standard liquid bottles 52a, 52b containing 5mg / L, 10mg / L, 25mg / L, 35mg / L, and 50mg / L respectively, and turn on the intake pump Switches 67a, 67b and peristaltic pump switches 68a, 68b, observe the readings of gas flow meters 43a, 43b, and manually adjust to an appropriate size; observe the readings on the peristaltic pump speed display 62 and adjust it to an appropriate size through the touch screen display . Operate the touch screen display to ...

Embodiment 3

[0089]Use this analyzer to test the BOD value of the river water in an artificial channel. The weather was fine that day, the temperature was 20°C, and the test location was the bank of the river. Only one channel is opened. For outdoor emergency testing, the standard curve has been established in advance, stored in the analyzer, and can be recalled on site. Open one way intake pump switch 67b and peristaltic pump switch 68b, observe the reading of gas flowmeter 43b, and manually adjust to a suitable size; observe the reading on the peristaltic pump speed display 62 and adjust it to a suitable size by touch screen display 63. Then put the measuring instrument liquid-taking tube 50b into the sample liquid bottle 53b to be tested, operate the touch display to the test mode, and after 8 minutes, the measuring instrument obtains that the BOD value of the sample liquid to be tested is 38 mg / L. The analyzer runs stably during the test, and is easy and safe to operate. The result c...

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Abstract

The invention relates to a double-channel microbial sensor type BOD (Biochemical Oxygen Demand) rapid tester for emergency detection. The tester is characterized in that a constant-temperature heatingunit is additionally provided with a water bath tank, and a tank high-limit liquid level sensor, a tank low-limit liquid level sensor, a tank water temperature sensor and a tank outlet liquid temperature sensor are arranged; two microbial sensor detection units are arranged and fixedly installed on a flip cover of the water bath tank, and an elastic flexible flat-bottom film protection disc witha disc bottom hole formed in the center is additionally arranged; two gas inlet and liquid inlet units are arranged, gas flow meters and liquid inlet peristaltic pumps capable of automatically adjusting the speed are additionally arranged on two gas inlet pipes, and a mixed gas-liquid inlet pipe enters the water bath tank and penetrates through water through a solenoid structure; and displays of an electrical control unit comprise a tank water temperature display, a touch screen display and a peristaltic pump rotating speed display, a buzzer for liquid level overrun alarming is additionally arranged, and an electrical control main board is a multifunctional control main board for controlling temperature, liquid level and operation. The tester has the beneficial effect that the requirementsof BOD emergency detection on rapidness, accuracy and safety are met.

Description

technical field [0001] The invention relates to an instrument device for measuring biochemical oxygen demand in water bodies, in particular to a dual-channel microbial sensor type rapid BOD detector for emergency detection. Background technique [0002] Biochemical Oxygen Demand (hereinafter referred to as BOD) is the amount of dissolved oxygen consumed by microbial metabolism, which is used to indirectly indicate the degree of water pollution by organic matter. Its definition is: within a certain period of time, The amount of free oxygen consumed by aerobic microorganisms to oxidize and decompose organic matter per unit volume of water, expressed in mg / L of oxygen, is one of the most important water quality detection indicators in environmental monitoring and pollution control. The main application It is used to detect the pollution of organic pollutants in water bodies. Because the BOD index value is the most direct for detecting and evaluating the pollution status of nat...

Claims

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Application Information

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IPC IPC(8): G01N27/31G01N27/416G01N33/18C12M1/38C12M1/36C12M1/12C12M1/04C12M1/00
CPCG01N27/31G01N27/4163G01N33/1806C12M23/02C12M29/06C12M41/12C12M41/44Y02A20/20
Inventor 王德龙闫志明刘和煦居文健李续李霞尹彦勋陈曦张玉惠王琳王悦
Owner 清池水环境治理科技发展(天津)有限公司
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