Preparation method of modified time-resolved fluorescent microspheres

A technology of time-resolved fluorescence and microspheres, applied in chemical instruments and methods, analytical materials, luminescent materials, etc., can solve the problems of no color of time-resolved microspheres and difficult test strips

Pending Publication Date: 2020-06-02
长沙美牛生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] However, time-resolved microspheres basically have no color (showing the white or light yellow color of latex microspheres). When using immunochromatography, users can only read the value through the instrument, and it is difficult to find abnormal test strips intuitively. The reason for the exception

Method used

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Examples

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Embodiment 1

[0022] In this embodiment, the steps are as follows:

[0023] Prepare sample 1:

[0024] Add 400ml of ultrapure water to the reactor, blow nitrogen for 30min~60min, stir while heating, the stirring speed is 270r / min, when the temperature rises to 70℃, add 20ml of rectified polystyrene (ST) together Mix and heat, then add 0.5 g of potassium persulfate and react for 6 hours to obtain white latex microspheres.

[0025] Weigh: 1 g of 2-thenoyl trifluoroacetone, 1 g of o-phenanthroline hydrochloride, and 0.5 g of europium chloride dissolved in 200 ml of petroleum ether, and then slowly add this mixture to the microsphere solution prepared above, Then it was stirred for 24 hours, and then the obtained time-resolved fluorescent microspheres were heated to evaporate all the petroleum ether. The synthesized microspheres were centrifuged at 10000r / min for 15min, the supernatant was discarded, 500ml ultrapure water was added, ultrasonic dispersion, and the above steps were repeated more than ...

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Abstract

The invention discloses a preparation method of modified time-resolved fluorescent microspheres. The preparation method comprises the following steps: (1) adding ultrapure water into a reaction kettle, introducing nitrogen, stirring while heating, adding rectified polystyrene, mixing and heating, then adding potassium persulfate, and fully reacting to obtain white latex spheres; and (2) weighing asolvent dye, 2-thiophene formyl trifluoroacetone, phenanthroline hydrochloride, europium chloride and an electron mediator, dissolving the solvent dye, the 2-thiophene formyl trifluoroacetone, the phenanthroline hydrochloride, the europium chloride and the electron mediator in petroleum ether to generate a mixture, slowly adding the mixture into a white latex microsphere solution prepared in step(1), uniformly stirring to obtain dyeing time-resolved fluorescent microspheres, heating to completely volatilize petroleum ether, and repeatedly centrifugally washing the synthesized microspheres tocompletely wash away redundant dyes in the solution in order to obtain the microspheres with bright colors and without influencing the time-resolved fluorescence of the microspheres. A user does notneed to read numerical values through an instrument when using the microspheres, and the reason of abnormality can be visually found out for an abnormal test strip.

Description

Technical field [0001] The invention relates to modified time-resolved fluorescent microspheres, in particular to a method for preparing modified time-resolved fluorescent microspheres. Background technique [0002] Time-resolved fluorescence has gradually become the mainstream of immunochromatographic detection because of its wide stoks shift and ultra-low background value. [0003] However, time-resolved microspheres have basically no color (showing the white or light yellow color of latex microspheres). When using immunochromatography, users can only read the values ​​through the instrument when using, and it is difficult to visually find abnormal test strips. The reason for the abnormality. [0004] How to provide a microsphere with bright colors and time-resolved fluorescence without affecting itself has become an urgent problem. Summary of the invention [0005] In order to solve the above-mentioned problems in the prior art, the present invention provides a method for prepari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06G01N33/558G01N33/58
CPCC09K11/06C09K11/025G01N33/582G01N33/585G01N33/558C09K2211/182
Inventor 石峰聂海龙
Owner 长沙美牛生物科技有限公司
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