Method for inducing formation of callus by using overwintering flower buds of lonicera fragrantissima

A callus and flower bud induction technology, applied in the field of plant biology, can solve the problem of limited plant materials

Active Publication Date: 2020-06-09
烟台市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Bitter candy is a small berry type plant, distributed sporadically in China, but less concentrated artificial planting, belongs to undeveloped wild plant germplasm resources, resulting in very limited plant materials for its breeding

Method used

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  • Method for inducing formation of callus by using overwintering flower buds of lonicera fragrantissima
  • Method for inducing formation of callus by using overwintering flower buds of lonicera fragrantissima
  • Method for inducing formation of callus by using overwintering flower buds of lonicera fragrantissima

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The formula used is MS+6-BA 0.5mg·L -1 +IBA 1mg·L -1 + sucrose 30g·L -1 + agar powder 9g·L -1 , and the other steps are the same operation method as in the summary of the invention for callus induction, and the callus of the plant material of the bitter candy cane is successfully induced.

Embodiment 2

[0036] The formula used is 1 / 2MS+6-BA 1.0mg·L -1 +IBA 0.5mg·L -1 + sucrose 30g·L -1 + agar powder 9g·L -1 , and the other steps are the same operation method as in the summary of the invention for callus induction, but the callus of the shoot tip plant material of bitter candy can not be successfully induced.

Embodiment 3

[0038] The formula used is 1 / 2MS+6-BA 0.5mg·L -1 +IBA 1.0mg·L -1 + sucrose 30g·L -1 + agar powder 9g·L -1 , and the other steps are the same operation method as in the summary of the invention for callus induction, but the callus of the shoot tip plant material of bitter candy can not be successfully induced.

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Abstract

The invention discloses a method discloses a method for inducing formation of callus by using overwintering flower buds of lonicera fragrantissima. The method comprises the following steps: removing outer skin of flower buds of overwintering branches of the lonicera fragrantissima to reserve yellowish green tender parts of the flower buds; and disinfecting the tender parts of the flower buds, andinoculating a culture medium with the tender parts of the flower buds, wherein the culture medium is an MS minimal culture medium with 6-BA hormone and/or IBA hormone, the mass ratio of the 6-BA hormone to the IBA hormone is 1:(1-2), the culture temperature is 26 +/- 1 DEG C, the illumination intensity is 4,000-6,000 lx, the illumination lasts for 12 h, and culture is carried out in a constant-temperature culture box for 15-25 days. The disinfection is carried out with alcohol, sodium hypochlorite, sterile water and mercuric chloride. By optimizing different hormone concentrations, the methodfor promoting formation of the callus of the overwintering flower buds with the overwintering flower buds of the lonicera fragrantissima with a test material is provided for the first time, and the blank of an asexual propagation manner of the lonicera fragrantissima is filled in.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for inducing callus formation by using bitter candy overwintering flower buds. Background technique [0002] Bitter candy (Lonicera fragrantissima Lindl. et Paxt. subsp. standishii (Carr.) Hsu et H.J. Wang) belongs to the subspecies of Lonicera genus Lonicera in the family Caprifoliaceae. This plant species is widely distributed in China, and mostly grows in sunny slopes or beside streams at an altitude of 100-2000m. Its fruit has high edible and nutritional value. [0003] In recent years, small berry plant species such as blueberry and wolfberry have been fully developed, creating good economic and social value. It is imperative to develop new small berry plant varieties and increase the market share of small berry. Bitter candy is a small berry type plant, distributed sporadically in China, but less concentrated artificial planting, belongs to undevelo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 苗杰陈甘牛赵莹路兆军
Owner 烟台市林业科学研究所
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