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Method for simultaneously and quantitatively detecting contents of epinephrine, phenothiazine and atropine drugs in sample

An epinephrine, quantitative detection technology, applied in the detection field, can solve the problems of long detection time, low accuracy, low efficiency, etc., and achieve the effects of good separation, accurate and reliable detection results, and simple preparation and operation.

Active Publication Date: 2020-06-12
CHINA INST OF VETERINARY DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] It can be seen from Table 1 that method four uses sodium thiosulfate titration, which is less specific and less accurate than HPLC, and is easily interfered by other compounds.
Because other methods detect the three types of ingredients separately, not only the detection time is long, the efficiency is low, and it is easy to miss the detection, and it is impossible to respond quickly to the illegal addition problem in the market, so it is not conducive to combating the illegal addition problem

Method used

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  • Method for simultaneously and quantitatively detecting contents of epinephrine, phenothiazine and atropine drugs in sample
  • Method for simultaneously and quantitatively detecting contents of epinephrine, phenothiazine and atropine drugs in sample
  • Method for simultaneously and quantitatively detecting contents of epinephrine, phenothiazine and atropine drugs in sample

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Embodiment 1

[0027] Embodiment 1. Simultaneous quantitative detection of the content of adrenergic receptor agonist drugs, phenothiazine drugs and atropine in the sample

[0028] 1. Experimental instruments and materials

[0029] High-performance liquid chromatography: American Waters Corporation, Waters2695 type, 2489 ultraviolet detector, Empower II workstation; chromatographic column: Agilent ZORBAX SB-C18 (4.6×250mm id.5μm), Thermo Hypersil Gold (4.6×250mm id.5μm) , Inertsil ODS-3 (4.6×250mm id.5μm), Waters Atlantis T3 C18 (4.6×250mm id.5μm);

[0030] Balance: Mettler AE240;

[0031] Chromatography: Waters 2695, PDA 2998;

[0032] pH meter: Mettler S210.

[0033] HILIC Silica (150×4.6mm id.5μm);

[0034] Test product: 0.9% sodium chloride injection, purchased from China Otsuka Pharmaceutical Co., Ltd., batch number 8183F6, as the sample to be tested;

[0035] Reference substance: see Table 2;

[0036] Table 2 Reference substance information

[0037] Reference product n...

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Abstract

The invention discloses a method for simultaneously and quantitatively detecting the contents of epinephrine, phenothiazine and atropine drugs in a sample. The method comprises the following steps oftaking a to-be-detected sample solution, detecting by the high performance liquid chromatography, and carrying out gradient elution by using a sodium heptanesulfonate solution as a mobile phase A andmethanol as a mobile phase B. According to the method, the sample is simple to prepare and operate, at most three or eight compounds can be detected at the same time, the separation condition betweenchromatographic peaks is good, and the method is suitable for detecting the content of epinephrine receptor stimulant drugs, phenothiazine drugs and atropine in food, drugs, blood products and the like.

Description

technical field [0001] The invention relates to the field of detection, in particular to a method for quantitatively detecting the contents of adrenoceptor agonist drugs, phenothiazine drugs and atropine in a sample simultaneously. Background technique [0002] Adrenergic receptor agonist drugs, such as epinephrine, norepinephrine bitartrate, isoproterenol, isoproterenol hydrochloride, phenylephrine hydrochloride, etc.; since dopamine is the precursor of norepinephrine, It will be metabolized into norepinephrine in the body. Therefore, like adrenergic receptor agonist drugs, it exerts its effect by stimulating the sympathetic nerve. Its pharmacological effects mainly include stimulating the cardiovascular system, relaxing bronchial smooth muscle, and promoting metabolism. Phenothiazine drugs, such as promethazine hydrochloride and chlorpromazine hydrochloride, act on dopamine receptors and have a strong inhibitory effect on the central nervous system. In large doses, they ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 于晓辉汪霞杨星董玲玲马秋冉韩宁宁顾进华
Owner CHINA INST OF VETERINARY DRUG CONTROL
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