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Method for rapid catalytic degradation of diethylstilbestrol

A technology of diethylstilbestrol and catalytic degradation, applied in the field of environmental pollution treatment, can solve the problems of the vicious cycle of environmental hormone pollution, the endocrine imbalance of humans and animals, threats to the reproduction and long-term survival of human and wild animal species, etc. low, high efficiency

Active Publication Date: 2020-06-19
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Diethylstilbestrol has the same physiological effect as natural estradiol, and is mainly used in clinical practice, but its hormone-like properties affect the normal hormone function in humans and animals, which can easily lead to endocrine imbalances in humans and animals, and diethylstilbestrol is fat-soluble Substances are easy to remain in animals, and even if they are excreted, they will accumulate in water and soil, causing a vicious cycle of environmental hormone pollution and seriously threatening the reproduction and long-term survival of humans and wild animals.

Method used

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  • Method for rapid catalytic degradation of diethylstilbestrol
  • Method for rapid catalytic degradation of diethylstilbestrol
  • Method for rapid catalytic degradation of diethylstilbestrol

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Add 0.5mL of 1mg / L laccase, 9.5mL of acetic acid buffer solution with pH5.5, and 200μL of 2.5g / L diethylstilbestrol into a 50mL Erlenmeyer flask and place it in a shaking incubator at 35°C for reaction;

[0034] (2) After reacting the solution in (1) for 0.5h, extract twice with ethyl acetate, collect the organic phase and evaporate it to dryness with a rotary evaporator, add 5mL of chromatographic grade methanol to constant volume, draw an appropriate amount with a 2mL syringe, pass 0.22 μm organic phase microporous filter membrane to the liquid phase sampling bottle, and finally use HPLC to detect the peak area;

[0035] (3) Calculate the remaining diethylstilbestrol concentration in the reaction system according to the peak area obtained by the detection and bring it into the standard curve of diethylstilbestrol. According to the formula degradation rate=(C 0 -C) / C 0 ×100% to calculate the degradation rate, where C 0 is the initial concentration, C is the rema...

Embodiment 2

[0037] (1) Add 0.5mL of 1mg / L laccase, 9.5mL of acetic acid buffer solution with pH5.5, and 200μL of 2.5g / L diethylstilbestrol into a 50mL Erlenmeyer flask and place it in a shaking incubator at 35°C for reaction;

[0038] (2) After reacting the solution in (1) for 1 hour, extract twice with ethyl acetate, collect the organic phase and evaporate it to dryness with a rotary evaporator, add 5 mL of chromatographic grade methanol to constant volume, draw an appropriate amount with a 2 mL syringe, and pass through 0.22 μm The organic phase microporous membrane was transferred to the liquid phase sampling bottle, and finally detected by HPLC, and the calculated degradation rate was 48.9%.

Embodiment 3

[0040] (1) Add 0.5mL of 1mg / L laccase, 9.5mL of acetic acid buffer solution with pH5.5, and 200μL of 2.5g / L diethylstilbestrol into a 50mL Erlenmeyer flask and place it in a shaking incubator at 35°C for reaction;

[0041] (2) After reacting the solution in (1) for 2 hours, extract twice with ethyl acetate, collect the organic phase and evaporate it to dryness with a rotary evaporator, add 5 mL of chromatographic grade methanol to constant volume, draw an appropriate amount with a 2 mL syringe, and pass through 0.22 μm The organic phase microporous membrane was transferred to the liquid phase sampling bottle, and finally detected by HPLC, and the calculated degradation rate was 58.6%.

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Abstract

The invention discloses a method for rapid catalytic degradation of diethylstilbestrol, and belongs to the technical field of environmental pollution treatment. The method comprises the following steps: dissolving diethylstilbestrol in methanol to form diethylstilbestrol mother liquor; dissolving laccase in water to form laccase mother liquor; diluting the diethylstilbestrol mother liquor into diethylstilbestrol standard solutions with different concentrations; mixing the diethylstilbestrol standard solution with the laccase mother solution, adding a buffer solution, extracting diethylstilbestrol with ethyl acetate, and terminating the reaction, wherein the reaction conditions are that the reaction temperature is 25-75 DEG C, the reaction pH is 3-8, and the degradation rate of a diethylstilbestrol solution with an initial concentration of 50 mg / L reaches 93.1% after the reaction is conducted for 6 h, the reaction is rapid, and secondary pollution is avoided.

Description

technical field [0001] The invention relates to the technical field of environmental pollution treatment, in particular to a method for rapidly catalytically degrading diethylstilbestrol. Background technique [0002] Diethylstilbestrol (DES) is a synthetic estrogen, which is one of the endocrine disruptors. Diethylstilbestrol has the same physiological effect as natural estradiol, and is mainly used in clinical practice, but its hormone-like properties affect the normal hormone function in humans and animals, which can easily lead to endocrine imbalances in humans and animals, and diethylstilbestrol is fat-soluble Substances are easy to remain in animals, and even if they are excreted, they will accumulate in water and soil, causing a vicious cycle of environmental hormone pollution and seriously threatening the reproduction and long-term survival of humans and wild animals. At present, diethylstilbestrol has been detected in animal-derived food and water quality, so the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/34
CPCC02F3/342C02F2101/345
Inventor 刘红艳谢世伟邓福兆唐琳林晓莲王秀丽单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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