Adult mouse myocardium separation device

Abstract

The invention relates to an adult mouse myocardium separation device. The device comprises a thermostat body, a peristaltic pump, an ice bath box body, an injection hose and a needle head fixer; wherein a culture dish placing area and a centrifuge tube placing area are embedded on upper surfaces of the ice bath box body and the thermostat body; the thermostat body is also provided with a temperature control device for heating and controlling temperature and a bracket for fixing the injection hose; the needle head fixer is connected with a syringe needle head; one end of the injection hose is connected with the needle head fixer; the peristaltic pump can be connected with the injection hose to realize circulation of liquid in the corresponding device; the culture dish placing area is used for placing a corresponding culture dish, the centrifuge tube placing area is used for placing a corresponding centrifuge tube, and a temperature detecting area is used for measuring temperature in thethermostat body. The device can not only provide a constant temperature experimental environment, but also provide a low temperature experimental environment, and facilitate transfer of experimentalobjects, can effectively control a perfusion speed of an isolated heart, simplify operation difficulty of an experiment, and accurately control an experiment process.

Description

technical field [0001] The invention relates to the technical field of myocardial cell separation technology, in particular, it is an adult mouse myocardial separation device. Background technique [0002] As a routine method in basic medical experiments, isolating rat cardiomyocytes is a key step in further research on the function of cardiomyocytes. The current method is to inject EDTA and protease to the left ventricle of the isolated myocardium through a syringe, but the temperature control and the pushing speed of the syringe will be affected by people, and the steps cannot exclude the variables of room temperature and human factors; and due to the limitations of the existing experimental equipment Limitations lead to cumbersome operations and are not convenient enough. [0003] Chinese patent document: 201310314848.7 discloses a method and device for acutely separating cardiomyocytes. During heart digestion during cardiomyocyte separation, a certain amount of lye is a...

AI Technical Summary

Problems solved by technology

The current method is to inject EDTA and protease to the left ventricle of the isolated myocardium through a syringe, but the temperature control and the pushing speed of the syringe will be affected by people, and the steps cannot exclude the variables of room temperature and human factors; and due to the limitations of the existing experimental equipment Limitation, resulting in cumbersome operation and not convenient enough

[0003] Chinese patent document: 201310314848.7 discloses a method and device for acutely separating cardiomyocytes. During heart digestion during cardiomyocyte separation, a certain amount of lye is added at regular intervals to adjust the pH of the digestive juice so that it can be stably maintained at around 7.40. It is consistent with the internal environment of the body; it can significantly improve the separation effect of cardiomyocytes, and at the same time eliminate the influence of heart volume, digestion time, and seasonal temperature on the separation effect, increase the stability of the separation effect, and obtain stable and consistent high-quality cardiomyocytes. This patent document Provides a set of perfusion device with controllable constant temperature and constant pH value, which can control the relative stability of the experimental environment, and provides a set of methods for acute separation of cardiomyocytes, but it solves the problem of pH value and temperature stability, the experimental environment It is relatively simple, and does not simplify the relevant experimental operations, nor does it solve the problem of complex transfer of experimental objects during the experimental process

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