Device and method for maintaining high viability of in vitro pollen of gramineous crops

Abstract

The invention relates to a device and method for maintaining high viability of in vitro pollen of gramineous crops. The device comprises a pollen collection chamber, a glass bead storage chamber, a desiccant storage chamber and a suction fan, wherein the glass bead storage chamber, the desiccant storage chamber and the suction fan are arranged at the upper end of the pollen collection chamber; thelower end of the pollen collection chamber is connected with a powder absorber; and the powder absorber is connected with a quantitative dry ice sprayer. According to the device for maintaining highviability of the in vitro pollen of the gramineous crops, crushed dry ice can be quantitatively mixed with air containing pollen when the pollen is collected to achieve the rapid sublimation cooling effect, the temperature of the pollen collection chamber is controlled at 7-15 DEG C all the time, refrigeration is rapid and uniform and the pollen collection time is prolonged, so that respiration ofthe pollen is inhibited through CO2 while high viability of the collected pollen is maintained, consumption of nutrients stored in the pollen is reduced, the viability of the pollen is maintained, the collection quality of the pollen is greatly improved and finally the setting rate of the pollen is improved.

Description

technical field [0001] The invention belongs to the technical field of pollen collection, and in particular relates to a device and a method for maintaining high vigor of isolated pollen of gramineous crops. Background technique [0002] After two varieties with different genetic properties are crossed, the hybrid generation will show very strong fecundity, vitality, stress resistance and adaptability, and will have more advantages than their parents in terms of yield and quality; pollen is sexual in plants. Plays an important role in reproduction. The genetic information of the paternal parent after pollen germination is the key to seed formation; pollen collection and storage and pollen vigor are the basis for seed reproduction and are also important links in male sterile line breeding and hybrid seed production. ; Before carrying out hybrid seed production of crops, it is necessary to collect a large amount of male parent material pollen for hybridization; in order to mee...

AI Technical Summary

Problems solved by technology

[0003] At present, pollen collection is mainly divided into manpower and machinery. The manual pollen collection method is not only labor-intensive, low in efficiency, and pollen collection is uneven, but also human operation may reduce the pollen vigor quickly, thereby reducing the seed production; while the current mechanical collection method During the pollination process, due to improper control of conditions such as difficulty in lowering the temperature during collection and long collection time, the viability of pollen will also be significantly reduced, seriously affecting the ability of pollen to fertilize and set fruit

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