Anti-browning callus induction culture medium for rhododendron longipedicellatum, and application thereof

A technology of callus induction and differentiation medium, which is applied in the fields of application, horticulture, botanical equipment and methods, etc. It can solve the problems of artificial destruction, low differentiation ability, callus browning, etc., and achieve the improvement of callus texture , Omit the steps of disinfection, reduce the effect of browning index

Inactive Publication Date: 2020-07-14
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the single distribution area in the wild, the scarcity of the population, and the severe human damage, it is already in a critically endangered state.
Therefore, it is of great significance to carry out the research on tissue culture and rapid propagation of Rhododendron long-stemmed, not only for future development and utilization, but also for the effective protection of this plant resource. However, the existing tissue culture system of Rhododendron long-stemmed has callus The problem of serious differentiation and low differentiation ability makes it difficult to obtain high-quality long-stemmed rhododendron tissue culture seedlings

Method used

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  • Anti-browning callus induction culture medium for rhododendron longipedicellatum, and application thereof
  • Anti-browning callus induction culture medium for rhododendron longipedicellatum, and application thereof
  • Anti-browning callus induction culture medium for rhododendron longipedicellatum, and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] The test materials were collected from Rhododendron longipedicellatum planted at the Experimental Station of Central Yunnan Plateau, Institute of Resource Entomology, Chinese Academy of Forestry.

[0034] 1. Effects of explant disinfection methods on callus induction and browning

[0035] Under aseptic conditions in the ultra-clean workbench, the long-stemmed rhododendron bracts were peeled off layer by layer, and immature flowers with a length of 0.6-1.2 cm were taken out as explants, and they were divided into two groups. The first group was directly inoculated without disinfection until induction medium. The explants of the second group (control) were surface-sterilized with 3% glutaraldehyde for 3 minutes, washed with sterile water for 4 times, then surface-sterilized with 0.1% mercury chloride for 3 minutes, washed with sterile water for 4 times, and inoculated until ferulic acid was added. in the callus induction medium. Control and treatment were each inoculate...

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Abstract

The invention provides an anti-browning callus induction culture medium for rhododendron longipedicellatum, and an application thereof, and relates to the technical field of plant tissue culture. Ferulic acid is used as an anti-browning agent in the culture medium of the invention, and when the concentration is 48.5 mg/L, ferulic acid does not significantly inhibit a growth amount of a callus, butcan significantly reduce the browning index of the callus. At the same time, when the culture medium is used to obtain a large number of differentiation buds or tissue culture seedlings of rhododendron longipedicellatum, a callus is inoculated into a differentiation culture medium containing the same concentration of ferulic acid, a differentiation rate of the callus can reach 17.9%, and therebythe bottleneck problem that tissue culture seedlings of new varieties of rhododendron longipedicellatum are difficult to obtain and thereby unable to be marketed is solved to a certain extent.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an anti-browning callus induction medium for Rhododendron long-stemmed and an application thereof. Background technique [0002] Long-stemmed Rhododendron is an evergreen plant of the genus Rhododendron, subg. Rhododendron, sect. Vireya, and subsect. Pseudovireya. Its flowers are thick in texture and bright yellow in color, compared with the rhododendron (R. rushforthii), rhododendron (R. molle), rhododendron (R. xanthostephanum), rhododendron (R. chrysodoron) and rhododendron sulfur in the same genus. (R.sulfureum) and other colors are more pure. Internationally, the flower color breeding trend of Rhododendron is pure color flowers, especially yellow and blue are more precious. What's even more amazing is that, unlike the wild rhododendrons, which usually bloom from March to June, the long-stemmed rhododendrons have a natural flowering period from late...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 万友名马宏李正红刘秀贤
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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