LncRNA-HOTAIR applied to treatment of osteoporosis

A technology for osteoporosis and osteoporosis, applied in the fields of molecular biology and medicine, can solve the problems of no reports on the treatment and application of osteoporosis, and achieve the effect of alleviating bone loss and increasing bone mass

Inactive Publication Date: 2020-07-24
63919 TROOPS PLA
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  • Description
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  • LncRNA-HOTAIR applied to treatment of osteoporosis
  • LncRNA-HOTAIR applied to treatment of osteoporosis
  • LncRNA-HOTAIR applied to treatment of osteoporosis

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experiment example 1

[0035] Experimental Example 1: Expression changes of lncRNA-HOTAIR during osteoblast differentiation

[0036] The experimental steps are as follows:

[0037] 1. Using the human pre-osteogenic cell line hFOB1.19 cells, using induction differentiation medium (DMEMF12 complete medium containing 50g / ml vitamin C, 2.0mM β-glycerophosphate sodium, 10nM dexamethasone) for osteotropic induction differentiation.

[0038]2. At fixed time points on days 0, 1, 2, 3, 4, and 5 of induction of differentiation, cells were collected with TriZol.

[0039] 3. Cell total RNA extraction steps:

[0040] Add chloroform to 200ul chloroform / ml Trizol for cell samples collected with Trizol for induced differentiation at 0, 1, 2, 3, 4, 5 days, vortex and mix well, place at room temperature for 15 min, centrifuge at 12000g for 15 min at 4°C, and absorb the upper layer of water Phase, into another centrifuge tube (note: try not to absorb the middle interface), add an equal volume of isopropanol, mix up...

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Abstract

The invention finds action of lncRNA-HOTAIR in osteoporosis. The lncRNA-HOTAIR is long-chain non-coding RNA, and the length of the lncRNA-HOTAIR is 2158nt. The long-chain non-coding RNA can be directly used for preparing reagents or medicines for detecting, preventing and/or treating osteoporosis diseases.

Description

[0001] field of invention [0002] The invention belongs to the fields of molecular biology and medicine, and relates to a novel lncRNA molecule that can be used for osteoporosis treatment and its application. The invention mainly relates to the application of a lncRNA molecule in the preparation of reagents and / or medicines for detecting, preventing and / or treating osteoporosis. Background technique [0003] Osteoporosis refers to a skeletal disease characterized by lower than normal bone mass per unit volume. Insufficient production of bone organic components, secondary calcium salt reduction, and micropore structure destruction of bone tissue are the main manifestations. Osteoporosis has become a worldwide problem, and it is another disease that seriously endangers human health after tumors and cardiovascular diseases. Studies have shown that the incidence of osteoporosis ranks seventh among common diseases and frequently-occurring diseases. [0004] Current status of ost...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883A61K31/7105A61P19/10
CPCC12Q1/6883A61K31/7105A61P19/10C12Q2600/158C12Q2600/178
Inventor 不公告发明人
Owner 63919 TROOPS PLA
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