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Labeled peptide of sheep-derived component and application of labeled peptide to detection of gelatin and gelatin products

A technology of labeling peptides and sheep origin, which is applied in the direction of measuring devices, material separation, and analysis materials, etc., can solve problems such as insurmountable difficulties, and achieve a strong conservative effect

Pending Publication Date: 2020-07-24
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned difficulties cannot be overcome by directly using liquid mass spectrometry to detect and analyze samples.

Method used

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  • Labeled peptide of sheep-derived component and application of labeled peptide to detection of gelatin and gelatin products
  • Labeled peptide of sheep-derived component and application of labeled peptide to detection of gelatin and gelatin products
  • Labeled peptide of sheep-derived component and application of labeled peptide to detection of gelatin and gelatin products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1 Materials and Reagents

[0018] Materials: Gelatin from different animal sources, respectively derived from sheep, goat, cattle, pig, horse, extracted from sheep, goat, cattle, pig, horse hide respectively.

[0019] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0020] 2 detection method

[0021] (1) Primary screening of labeled peptides

[0022] Through multiple batches of mRNA comparison experiments of various animal COL1A2 proteins, it shows that among the three bases corresponding to each amino acid, the first base A and T, and the second base C and T are basically different in the same species. Mutations occur frequently between species. The goat COL1A2 protein mRNA contains "GGC CCT GCT GGT CCT ACT GGC CCC GCT GGCAAA"; in the corresponding position, the sheep is "GGC CCC GCT GGT CCT ACT GGC CCC GCT GGC AAA", and the cow is "GGC CCT GCT GGT CC...

Embodiment 2

[0029] 1 Materials and Reagents

[0030] Material: mixed gelatin sample (comprising: the bovine gelatin containing 5% sheep gelatin, the bovine gelatin of 10% sheep gelatin, the bovine gelatin of 20% sheep gelatin, the bovine gelatin of 40% sheep gelatin, pure sheep gelatin) by the embodiment 1 Made with a precise blend of gelatin.

[0031] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0032] 2 detection method

[0033] (1) Take 0.05g gelatin sample in a 25ml measuring bottle, add a small amount of 1% NH 4 HCO 3 Soak the solution for 10 minutes, heat it at 60°C to dissolve it, and use 1% NH 4 HCO 3 Dilute the solution to the mark, shake well, filter with a microporous membrane, accurately measure 0.2ml of the subsequent filtrate, add 20μl of 2mg / ml trypsin solution, and enzymolyze at a constant temperature of 37°C for 12h.

[0034] (2) Take 5 μl of the e...

Embodiment 3

[0037] 1 Materials and Reagents

[0038] Ingredients: sheep gelatin, QQ sugar with pig gelatin (homemade), QQ sugar with sheep gelatin (homemade)

[0039] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0040] 2 detection method

[0041] (1) Take 0.05-0.25g of sheep gelatin, QQ sugar with pig gelatin, and QQ sugar with sheep gelatin, put them in 25ml measuring bottles, add a small amount of 1% NH 4 HCO 3 Soak the solution for 10 minutes, heat it at 60°C to dissolve it, and use 1% NH 4 HCO 3 Dilute the solution to the mark, shake well, filter with a microporous membrane, accurately measure 0.2ml of the subsequent filtrate, add 20μl of 2mg / ml trypsin solution, and enzymolyze at a constant temperature of 37°C for 12h.

[0042] (2) Take 5 μl of the enzymatic hydrolysis solution and put it into the liquid mass spectrometer for detection. Liquid phase condition:...

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Abstract

The invention discloses a labeled peptide of a sheep-derived component and application of the labeled peptide to detection of gelatin and gelatin products, and belongs to the technical field of detection. A labeled peptide, namely Gly-Pro-Ala-Gly-Pro-Thr-Gly-Pro-Ala-Gly-Lys of the sheep-derived component is involved, and whether the sheep-derived component is contained or not can be determined bydetecting the labeled peptide, specifically, pancreatin digestion is carried out on protein components in a sample to dissociate the labeled peptide, and then whether the labeled peptide is containedor not is detected through a liquid chromatograph-mass spectrometer to judge whether the sheep-derived component is contained or not. A method is strong in characteristic, high in sensitivity and easyto operate, and can determine the sheep-derived component in the gelatin and gelatin products.

Description

technical field [0001] The invention relates to a labeled peptide of sheep-derived components and its application in the detection of gelatin and gelatin products, belonging to the field of medicine and food detection. Background technique [0002] Gelatin is made from animal bones, skins, tendons, tendons and scales through moderate hydrolysis. It has excellent physical and chemical properties and is widely used in many fields such as food and medicine. Gelatin extracted from different animals often has different uses; more importantly, due to the possible safety risks caused by animal diseases, halal requirements and religious beliefs, it is necessary to trace the animal-derived components of gelatin. 95% of gelatin in the world comes from pigs, cattle or sheep, so it is of great significance to trace the source of sheep-derived ingredients. [0003] The main component of gelatin is collagen polypeptide, which can be traced to the source of animal-derived components by de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7266G01N30/8637
Inventor 郭尚伟黄雅钦
Owner BEIJING UNIV OF CHEM TECH
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