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Chicken-derived characteristic III type collagen peptide and application thereof in detection of collagen hydrolysate and products thereof

A collagen peptide, chicken-derived technology, used in specific peptides, biological testing, measuring devices, etc., can solve problems such as insurmountable difficulties, and achieve accurate traceability detection and strong conservative effects.

Active Publication Date: 2020-11-06
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned difficulties cannot be overcome by directly using liquid mass spectrometry to detect and analyze samples.

Method used

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  • Chicken-derived characteristic III type collagen peptide and application thereof in detection of collagen hydrolysate and products thereof
  • Chicken-derived characteristic III type collagen peptide and application thereof in detection of collagen hydrolysate and products thereof
  • Chicken-derived characteristic III type collagen peptide and application thereof in detection of collagen hydrolysate and products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1 Materials and Reagents

[0025] Materials: Gelatin from different animal sources, from chicken, duck, cow, pig, and sheep, and extracted from chicken skin, duck skin, cowhide, pig skin, and sheepskin.

[0026] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0027] 2 Screening and determination of characteristic peptides

[0028] After testing chicken skin, it was found that chicken skin contains a large amount of COL3A1 protein, and this protein has a certain degree of conservation, so a peptide can be screened from COL3A1 protein as a characteristic peptide of chicken-derived ingredients. In order to screen a stable characteristic peptide for detection from a gene, the following conditions should be met: (a) The three bases adjacent to the mRNA sequence corresponding to the peptide should be arginine or lysine after translation , and the first to thi...

Embodiment 2

[0037] Same method as in Example 1.

[0038] 1 Materials and Reagents

[0039] Materials: Gelatin from different animal sources, from chicken, duck, cow, pig, and sheep, and extracted from chicken skin, duck skin, cowhide, pig skin, and sheepskin.

[0040] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0041] 2 detection method

[0042] (1) Preliminary screening of characteristic peptides

[0043] Compare the mRNA sequences corresponding to the COL3A1 proteins of chicken, duck, cow, pig, and sheep, and the partial sequences are shown as Figure 9 (Note: mRNA is transcribed from DNA in the nucleus, and sometimes the sequence of bases on the DNA chain is used to represent the sequence on the mRNA). Wherein the chicken mRNA sequence "GGC AGC CCA GGT CCC CCT GGC CCA AGT GGA CCT GCGGGA GAC CGT" in the wire frame satisfies the conditions (a), (b) and (c) in Examp...

Embodiment 3

[0050] 1 Materials and Reagents

[0051] Material: mixed gelatin samples (comprising: pig gelatin containing 5% chicken gelatin, pig gelatin of 10% chicken gelatin, pig gelatin of 20% chicken gelatin, pig gelatin of 40% chicken gelatin, pure chicken gelatin) by the embodiment 1 Made with a precise blend of gelatin.

[0052] Reagents: ammonium bicarbonate (analytical pure), trypsin (serial pure, purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0053] 2 detection method

[0054] (1) Take 0.05g gelatin sample in a 25ml measuring bottle, add a small amount of 1% NH 4 HCO 3 Soak the solution for 10 minutes, heat it at 60°C to dissolve it, and use 1% NH 4 HCO 3 Dilute the solution to the mark, shake well, filter with a microporous membrane, accurately measure 0.2ml of the subsequent filtrate, add 20μl of 2mg / ml trypsin solution, and enzymolyze at a constant temperature of 37°C for 12h.

[0055] (2) Take 5 μl of the enzymatic hydrolys...

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Abstract

The invention discloses a chicken-derived characteristic III type collagen peptide and application thereof in detection of collagen hydrolysate and products thereof, which belong to the technical field of detection. Characteristic III type collagen peptide amino acid sequences of the chicken-derived components are Gly-Ala-Gln-Gly-Pro-Pro-Gly-Pro-Thr-Gly-Ala-Arg and Gly-Ser-Hyp-Gly-Pro-Hyp-Gly-Pro-Ser- Gly-Pro-Ala-Gly-Asp-Arg. By detecting one or two peptides, whether the chicken-derived component is contained or not is determined. Pancreatic enzyme digestion on protein components in a sample is carried out to dissociate characteristic III type collagen peptide, whether the characteristic III type collagen peptide is contained or not is detected by using a liquid chromatograph-mass spectrometer, and whether the chicken-derived components are contained or not is judged. The method has the advantages of strong characteristics, high sensitivity and simple operation, and can be used for determining the chicken-derived components in the collagen hydrolysate and products thereof.

Description

technical field [0001] The invention relates to chicken-sourced characteristic type III collagen peptide and its application in the detection of collagen hydrolyzate and its products, and belongs to the field of medicine and food detection. Background technique [0002] Collagen is very abundant in animals, and mainly exists in connective tissues such as bones, skin, tendons and tendons of animals. Gelatin is a hydrolyzate of collagen, which is obtained by moderate hydrolysis of animal bones, skins, tendons and tendons. It has excellent physical and chemical properties and is widely used in many fields such as food and medicine. Gelatin is mostly made of skin or bone of pigs, cattle and sheep. However, due to possible safety risks caused by diseases of poultry and livestock, and differences in halal and religious beliefs, it is of great significance to trace the source of animal-derived components of gelatin. [0003] The main component of gelatin is collagen peptide, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/72G01N30/86G01N30/88G01N30/89G01N30/14C07K14/78C12P21/06G01N33/68
CPCG01N30/06G01N30/72G01N30/8634G01N30/88G01N30/89G01N30/14C07K14/78C12P21/06G01N33/68G01N2030/067G01N2030/8831G01N2030/8818G01N2030/146G01N2333/78
Inventor 郭尚伟黄雅钦
Owner BEIJING UNIV OF CHEM TECH
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