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A gene cell-free dna extraction system

An extraction system and gene technology, applied in the field of gene free DNA extraction system, can solve cumbersome problems, achieve stable control, realize short-distance transportation, and facilitate extraction work

Active Publication Date: 2020-10-27
杭州同创医学检验实验室有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the process of nucleic acid extraction, a shaking table is often used for vibration, and it needs to cooperate with the magnetic frame for transfer and transportation many times, which is cumbersome; therefore, it is necessary to develop a more comprehensive system to complete the complete process of physical shaking and transfer and transportation

Method used

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  • A gene cell-free dna extraction system
  • A gene cell-free dna extraction system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] Embodiment: This embodiment takes the existing tumor mutation gene detection process as an example:

[0026] Including sample extraction system: use streck tube to collect 10ml of blood, store and transport at 10℃~30℃; or use EDTA anticoagulant tube to collect 10ml of blood, separate plasma within 2 hours, store and transport plasma sample to testing laboratory at -80℃;

[0027] Sample Lysis System: Add each component in sequence in a 15ml centrifuge tube: protease; plasma sample; sample lysate;

[0028] Including a test tube rack 11;

[0029] refer to figure 1 as well as figure 2 As shown: the bottom of the test tube rack 11 is provided with a support rod 2, and the bottom of the support rod 2 is fixedly connected with a worm wheel 21, and the worm wheel 21 is engaged between two worms 1, and the two worms 1 The structures are the same, and each of the worms 1 is independently driven by a motor;

[0030] The middle section of the support rod 2 is provided with a w...

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Abstract

The invention discloses a gene free DNA extraction system. According to the gene free DNA extraction system, collecting of 10ml of blood is carried out by using a streck tube, and storing and transporting are performed at 10-30 DEG C; a sample cracking system is used for sequentially adding the following components into a 15ml test tube: protease, a plasma sample and a sample cracking solution; atest tube rack is included, wherein a supporting rod is arranged at the bottom of the test tube rack; a turbine is fixedly connected to the bottom of the supporting rod; the turbine is meshed betweentwo worms, the two worms are of the same structure, and each worm is independently driven by a motor; a worm part is arranged in the middle section of the supporting rod, gears are connected to the outer side of the worm part in a meshed mode, and each gear is fixedly connected with a supporting arm; and the supporting arms are connected with the test tube rack through hinges. The extraction system innovatively provides a nucleic acid extraction system, which can realize oscillation and short-range transportation, is stable and reasonable in control, and greatly facilitates the extraction workof nucleic acid.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a gene free DNA extraction system. Background technique [0002] Life science is an experimental science. A large number of biological experiments and clinical diagnosis are inseparable from sample preparation. Nucleic acid extraction is widely used in sample preparation. With the development of biotechnology, the number of samples processed in clinical diagnosis and biological experiments is often very large. The traditional nucleic acid preparation method is to add the sample to be tested into a reagent containing a chemical reagent or lyase, and achieve the preparation of the nucleic acid sample through a chemical reaction. [0003] In the process of nucleic acid extraction, a shaking table is often used for vibration, and it needs to cooperate with the magnetic frame for transfer and transportation many times, which is cumbersome; therefore, it is necessary t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/42C12M1/36C12M1/24C12N15/10
CPCC12N15/1013
Inventor 许亚丽赵俊生袁慧兴余嘉伟洪亚妮
Owner 杭州同创医学检验实验室有限公司
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