Compositions and methods for improved gamete viability and function

A technology for compositions and formulations, applied in biochemical equipment and methods, applications, microorganisms, etc., to solve problems such as not allowing sufficient maintenance of sperm, interfering with downstream use of sperm, etc.

Pending Publication Date: 2020-07-31
ABS GLOBAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commercial media may not allow adequate maintenance of sperm viability and / or activity
Additionally, commercially available media may interfere with the downstream use of sperm, such as during sex sorting or in IVF or AI

Method used

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  • Compositions and methods for improved gamete viability and function
  • Compositions and methods for improved gamete viability and function
  • Compositions and methods for improved gamete viability and function

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0123] Sexing procedures require sperm to survive substantial damage, and while preliminary data demonstrate that SVFEM Extender promotes successful sexing up to 36 hours after collection, the fertilization capacity of expanded sperm has not yet been assessed. Although scientists have attempted to correlate in vitro sperm characteristics with fertilization outcomes, no such assay has yet become a gold standard. Cellular assessments of plasma membranes, intracellular structures, and testing of velocity parameters with the CASA system or with the bovine cervical mucus penetration test (BCMPT) have often given conflicting results regarding their relationship to IVF or AI fertility. For example, plasma membrane PI staining integrity correlated with field fertility for Januskauskas et al. (2003) but not for Oliveira et al. (2012). Furthermore, distance traveled in BCMPT appears to correlate with NRR (Tas et al., 2007; Bacinoglu et al., 2007), but this measure does not correlate str...

example 2

[0169] Ejaculates from two sires were processed as split ejaculates, with one half yielding sexed control semen and the other half yielding semen sexed after 24 hr SVFEM expansion. The relative conception rate is reported as [(SVFEM extended pregnancy %) / (control pregnancy %)]*100.

[0170] For sire 1, the relative SVFEM / control conception rate (relative conception rate) was 92%.

[0171] For sire 2, the relative conception rate was 126%.

[0172] The average between the two is 109%.

[0173] This data indicates that pregnancy can be achieved with 24 hour SVFEM expanded semen.

[0174] Initial data points, conception rates appeared similar to the controls.

example 3

[0176]

[0177] Table 2: Pearson's correlation coefficient values ​​correlating IVF results with velocity measurements taken after thawing in the test dilution medium. Those highlighted indicate that the slope value is significantly different from zero at a = 0.05. Reported R 2 is the adjusted R 2 value.

[0178] Medium formula:

[0179] All chemicals were purchased through Sigma-Aldrich unless otherwise stated.

[0180] Tyrode's solution (Tyrode's): 1 liter H 2 o

[0181] 94.5mM NaCl

[0182] 3mM KC1

[0183] 300 μM Na 2 HPO 4

[0184] 10mM NaHCO 3

[0185] 400 μM MgCl 2 *6H 2 o

[0186] Osmolality 180 to 190mOsm

[0187] Filter with a 0.22 μm bottle top PTFE filter

[0188] Stain TALP:

[0189] 1 liter of Tyrode's solution

[0190] 25mM Sodium Lactate Syrup (60% w / w)

[0191] 5mM Glucose 40mM HEPES

[0192] 0.5mM sodium pyruvate

[0193] 3mg / mL BSA (fraction V)

[0194] pH 7.6

[0195] Osmolality 290 to 320mOsm

[0196] Filter with a 0.22 μm bottl...

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Abstract

The present specification provides for a media composition that provides enhanced viability and activity to sperm cells.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Application No. 62 / 578,959, filed October 30, 2017, the contents of which are incorporated herein in their entirety. technical field [0003] This disclosure relates to the field of animal farming and breeding. In particular, the present disclosure encompasses improved medium formulations for use with ejaculate and sperm cell samples and for use during fertilization. Such medium formulations provide improved sperm cell motility, enhanced sperm cell function (including motility and fertility), and enhanced zygote formation (ie, fertilization). Background technique [0004] An important aspect of animal breeding, especially in agriculture, is the collection and use of sperm cells (spermatozoa). Typically, sperm cells are collected in the form of raw ejaculated semen from a male animal. Subsequent use and manipulation of sperm cells requires maintenance of cell viab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/076
CPCA01N1/021A01N1/0226C12N5/061C12N2500/16C12N2500/14A01N1/0284C12N5/0018
Inventor E·C·罗蒂罗蒂
Owner ABS GLOBAL
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