Tissue culture speed propagation method for american eleutherine rhizome

Abstract

The invention provides a tissue culture speed propagation method for american eleutherine rhizome. The method comprises the steps of cutting off root hair and american eleutherine rhizome leaves at amorphological upper end of a plant of american eleutherine rhizome; stripping several layers of leaves outside a pseudostem, and carrying out washing to obtain an initial explant material; disinfecting the initial explant material with an ethanol solution and a mercury bichloride solution, carrying out washing and then stripping external leaves; when a shiny hemispherical tissue is seen, cutting out tip meristems with 2-3 leaf primordia, putting the tip meristems into a primary medium to culture to obtain bud points which turn green and grow up; culturing the bud points in a cluster bud induction medium, carrying out segmentation by taking 2-3 cluster buds as one clump, transferring the cluster buds into a proliferation medium to culture to obtain seedlings, and transferring the seedlingsinto a rooting medium to culture; and placing obtained rooted plantlets in scattered light, and carrying out washing, transplanting and culturing to obtain the american eleutherine rhizome. The american eleutherine rhizome obtained by the method is high in propagation coefficient, the problem of a low propagation coefficient of the american eleutherine rhizome caused by tillering and aerial shallot propagation in a conventional method can be solved, and the tissue culture speed propagation method is not limited to seasons, and high in practical applicability.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of shallots. Background technique [0002] "Red onion" is a variety of Allium onion in the Liliaceae family. Its unique and strong onion flavor is especially loved by people in the northwest region who like to eat mutton. The pseudostem does not swell obviously, and when it is mature, it turns into a brown red onion coat to wrap the pseudostem, hence the name "red onion". The flower stem is cylindrical and hollow, and its characteristic is that there are no seeds on the flower stem, but aerial shallots are produced by flower organs at the top of the flower stem. Conventional propagation methods use aerial shallots on tillers and flower stems to reproduce, and the reproduction coefficient is low, which cannot meet the needs of people's production and life. Contents of the invention [0003] The technica...

AI Technical Summary

Problems solved by technology

Conventional propagation methods use aerial shallots on tillers and flower stems to reproduce, and the reproduction coefficient is low, which cannot meet the needs of people's production and life.

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