Universal animal sperm sorting method and X sperm quality control method

A quality control method and a general-purpose technology, applied in the biological field, can solve the problems of sperm damage, expensive equipment, gene teratogenicity, etc., and achieve the effect of small sperm damage, high accuracy, and reduced impact

Pending Publication Date: 2020-08-07
YANAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also unavoidable problems in the use of flow cytometry separation, such as the teratogenic effect

Method used

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  • Universal animal sperm sorting method and X sperm quality control method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0074] A general-purpose animal sperm sorting method, specifically according to the following steps:

[0075] Step 1, collect the semen of the animal to be sorted, mix it with the sperm diluent, put it into a storage bottle after mixing evenly, and place the storage bottle in an incubator with a temperature of 20°C;

[0076] Step 2, add the sorting solution into the storage bottle, let it stand for 1 hour and then separate the layers, use a water pump to pump out the upper layer solution, the upper layer solution contains Y sperm, and the remaining lower layer solution contains X sperm;

[0077] Step 3, adding the extraction solution to the lower layer solution, extracting the X sperm from the sorting solution, and distributing them in 100 mL sub-package bottles to obtain a solution containing the X sperm.

[0078] In step 1, sperm diluent is made of 12% sucrose solution, glycine, sodium citrate, Tris, egg yolk, glycerin and penicillin streptomycin, add 0.5g glycine, 0.5g lemo...

Embodiment 2

[0095] A general-purpose animal sperm sorting method, specifically according to the following steps:

[0096] Step 1, collect the semen of the animal to be sorted, mix it with the sperm diluent, put it into a storage bottle after mixing evenly, and place the storage bottle in an incubator with a temperature of 15°C;

[0097] Step 2, add the sorting solution into the storage bottle, let it stand for 1 hour and then separate the layers, use a water pump to pump out the upper layer solution, the upper layer solution contains Y sperm, and the remaining lower layer solution contains X sperm;

[0098] Step 3, adding the extract solution to the lower layer solution, extracting X sperm from the sorting solution, and distributing them in 100mL sub-package bottles to obtain a solution containing X sperm;

[0099] In step 1, sperm diluent is made of 12% sucrose solution, glycine, sodium citrate, Tris, egg yolk, glycerin and penicillin streptomycin, add 0.5g glycine, 0.5g lemon to 75ml of...

Embodiment 3

[0116] A general-purpose animal sperm sorting method, specifically according to the following steps:

[0117] Step 1, collect the semen of the animal to be sorted, mix it with the sperm diluent, put it into a storage bottle after mixing evenly, and place the storage bottle in an incubator with a temperature of 18°C;

[0118] Step 2, add the sorting solution into the storage bottle, let it stand for 1 hour and then separate the layers, use a water pump to pump out the upper layer solution, the upper layer solution contains Y sperm, and the remaining lower layer solution contains X sperm;

[0119] Step 3, adding the extraction solution to the lower layer solution, extracting the X sperm from the sorting solution, and distributing them in 100 mL sub-package bottles to obtain a solution containing the X sperm.

[0120] In step 1, sperm diluent is made of 12% sucrose solution, glycine, sodium citrate, Tris, egg yolk, glycerin and penicillin streptomycin, add 0.5g glycine, 0.5g lemo...

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Abstract

The invention discloses a universal animal sperm sorting method which is characterized by comprising the following specific steps: collecting a seminal fluid of an animal to be sorted, mixing the seminal fluid with sperm diluent, putting the mixture into a storage bottle after uniform mixing, and putting the storage bottle into a heat preservation box with the heat preservation temperature of 15-20 DEG C, 2, adding the sorting liquid into the storage bottle, standing for 1 h, then layering, by a water pumping device, pumping out an upper-layer solution containing Y sperms, and the remaining the lower-layer solution containing X sperms and 3, adding an extracting solution into the lower-layer solution, extracting the X sperms from the sorting liquid, and sub-packaging in 100 mL of sub-packaging bottles to obtain a solution containing X sperms. The invention further discloses an X sperm quality control method, a series of operations such as sperm collection, sperm dilution, sperm sorting, sperm extraction and sperm quality control can be effectively completed, and the X sperm quality control method is suitable for livestock sex control in animal husbandry and breeding industry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a general animal sperm sorting method, and also relates to a X sperm quality control method. Background technique [0002] Sex control refers to the technology of making female breeding animals breed offspring of the desired sex according to people's wishes through human intervention. Sex control technology is of great significance in livestock production. First of all, the sex control technology of livestock is a bioengineering technology that can significantly increase the reproductive rate of livestock. Controlling the sex ratio of offspring can increase the intensity of selection and speed up the breeding process, which is very important for livestock breeding, production and the prevention and treatment of genetic diseases. meaning. Secondly, the sex control technology of domestic animals is also an important reproductive technology in the production of animal husbandry and bree...

Claims

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Application Information

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IPC IPC(8): C12N5/076C12Q1/6844C12Q1/06
CPCC12N5/061C12Q1/6844C12N2509/00C12Q2531/119C12Q2563/107
Inventor 岳昌武吕玉红崔相宜周进科孙心悦王雪梅郭瑞瑞
Owner YANAN UNIV
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