Rapid sulfadiazine detection method

A sulfadiazine and detection method technology, applied in the field of rapid biological detection, can solve the problems of difficulty in meeting the requirements of rapid sample screening, long pretreatment process time, expensive instruments and equipment, etc., and achieve the effect of a fast and convenient detection method

Pending Publication Date: 2020-08-25
涂坤
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Problems solved by technology

Chromatography and mass spectrometry generally have defects such as long pretreatment process, complicated and technical operation, and expensive instruments and equipment, which make it difficult to meet the requirements for rapid screening of a large

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  • Rapid sulfadiazine detection method
  • Rapid sulfadiazine detection method
  • Rapid sulfadiazine detection method

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Embodiment Construction

[0028] In order to express the present invention more clearly, the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0029] Surface-enhanced Raman spectroscopy refers to the use of gold and silver nanoparticles as carriers. When the molecules to be tested are adsorbed on the rough gold and silver surfaces, the surface area between light and molecules will be greatly increased, and the electromagnetic field on the metal surface will also increase. The amplitude is enhanced, and the obtained Raman signal strength is ordinary 10 6 more than double. Surface-enhanced Raman spectroscopy has the advantages of simple pretreatment of experimental samples, fast detection of a single sample, and high detection sensitivity. Therefore, it has been widely used in the rapid monitoring of quality and safety issues such as trace pesticide residues and antibiotic drug residues in food and agricultural products. in the state of th...

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Abstract

The invention provides a rapid detection method of sulfadiazine in an animal body, and a Raman characteristic peak of sulfadiazine is determined by using a density functional theory. The method comprises a step of establishing a quantitative analysis curve according to the characteristic peak intensity with high Raman peak intensity and a good peak pattern. Standard recovery experiments show thatthe method is good in accuracy and precision. The lowest concentration of sulfadiazine in the animal body detected by the method is 2 mg/L. According to the method, the sulfonamide antibiotic residuesin the swine urine are qualitatively and quantitatively analyzed, single sample detection is completed within 3 min, and a rapid and convenient detection method is provided for rapid qualitative screening and preliminary quantitative detection of the sulfonamide antibiotic residues in the swine urine.

Description

technical field [0001] The invention relates to the field of rapid biological detection, in particular to a rapid detection method for sulfadiazine. Background technique [0002] Veterinary drug residues refer to the parent compound and / or its metabolites of veterinary drugs contained in any edible part of animal products, as well as impurities related to veterinary drugs. These include antibiotics, anthelmintics, antiprotozoals, trypanosomidates, etc. [0003] Veterinary drugs and feed additives left in animal-derived food enter the human body along the food chain, posing a potential threat to human health. This threat has attracted more and more attention. With the change of people's demand for animal-derived food, the international requirements for drug residues in animal-derived food are getting higher and higher. Drug residues in animal-derived food have gradually become a focus of attention worldwide, among which antibiotic residues are subject to Great attention. T...

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 涂坤
Owner 涂坤
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