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Gel matrix based on dopamine grafted zwitterionic polyphosphorylcholine modified type I collagen and preparation method of gel matrix

A zwitterion and phosphorylcholine technology, applied in biochemical equipment and methods, bone/connective tissue cells, animal cells, etc., can solve the insufficient mechanical properties of a single type I collagen matrix, affect the effect of cartilage repair, and anti-inflammatory factors Poor performance and other problems, to achieve good chondrocyte migration and growth performance, good resistance to non-specific adsorption performance, and overcome insufficient lubrication performance

Pending Publication Date: 2020-09-04
ARTHRO ANDA TIANJIN BIOLOGIC TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties of a single type I collagen matrix are insufficient, and the performance of anti-inflammatory factors is poor, which seriously affects the effect of repairing cartilage after implantation.

Method used

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  • Gel matrix based on dopamine grafted zwitterionic polyphosphorylcholine modified type I collagen and preparation method of gel matrix
  • Gel matrix based on dopamine grafted zwitterionic polyphosphorylcholine modified type I collagen and preparation method of gel matrix
  • Gel matrix based on dopamine grafted zwitterionic polyphosphorylcholine modified type I collagen and preparation method of gel matrix

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1) Preparation of dopamine-grafted zwitterionic polymerized phosphorylcholine (DOPA-B):

[0036] Prepare 10mM phosphate buffer at pH=7.4 with disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate. Take 1.2g of zwitterionic polymerized phosphorylcholine (B) and dissolve it in 120mL of the above-mentioned phosphate buffer solution, adjust the pH of the solution to 5 with 1.0M hydrochloric acid, and then add 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (the molar ratio of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and zwitterionic polymeric phosphorylcholine repeating unit is 2:1) After stirring for 20 min, dopamine was added (the molar ratio of dopamine and zwitterionic polymeric phosphorylcholine repeating units was 1:1), the pH of the solution was maintained at 5 with 1M hydrochloric acid, and the reaction was stirred at 20°C for 1 hour. The reacted solution was placed in a dialysis bag (MWCO: 8000-14000 Da), and di...

Embodiment 2

[0063] 1) Preparation of dopamine-grafted zwitterionic polymerized phosphorylcholine (DOPA-B):

[0064] Prepare 10mM phosphate buffer at pH=7.4 with disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate. Dissolve 1.2g of zwitterionic polymerized phosphorylcholine (B) in 120mL of the above phosphate buffer, adjust the pH of the solution to 5.5 with 1.0M hydrochloric acid, and then add 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (the molar ratio of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and zwitterionic polymeric phosphorylcholine repeating unit is 2:1) After stirring for 25 min, dopamine was added (the molar ratio of dopamine and zwitterionic polymerized phosphorylcholine repeating unit was 2:1), and the pH value of the solution was maintained at 5.5 with 1M hydrochloric acid, and the reaction was stirred at 25°C for 2 hours. The reacted solution was placed in a dialysis bag (MWCO: 8000-14000 Da), and dialyzed in an aq...

Embodiment 3

[0077] 1) Preparation of dopamine-grafted zwitterionic polymerized phosphorylcholine (DOPA-B):

[0078] Prepare 10mM phosphate buffer at pH=7.4 with disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate. Dissolve 1.2g of zwitterionic polymeric phosphorylcholine (B) in 120mL of the above phosphate buffer, adjust the pH of the solution to 6 with 1.0M hydrochloric acid, and then add 1-ethyl-(3-dimethyl Aminopropyl) carbodiimide hydrochloride (the molar ratio of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and zwitterionic polymeric phosphorylcholine repeating unit is 2:1) After stirring for 30 min, dopamine was added (the molar ratio of dopamine and zwitterionic polymerized phosphorylcholine repeating units was 3:1), and the pH value of the solution was maintained at 6 with 1M hydrochloric acid, and the reaction was stirred at 30°C for 3 hours. The reacted solution was placed in a dialysis bag (MWCO: 8000-14000 Da), and dialyzed in an aqueous...

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Abstract

The invention relates to a gel matrix based on dopamine grafted zwitterionic polycholine modified type I collagen and a preparation method of the gel matrix, and belongs to the technical field of biological materials. The novel gel matrix comprises modified zwitterionic polyphosphorylcholine and modified type I collagen. The preparation method comprises the following steps: firstly, synthesizing dopamine grafted modified zwitterionic polyphosphorylcholine through amidation reaction; and connecting zwitterionic polymerized phosphorylcholine to the surface of an I-type collagen matrix through the adhesion during dopamine oxidative polymerization so as to obtain the novel gel matrix based on dopamine grafted zwitterionic polymerized phosphorylcholine modified I-type collagen. The novel gel matrix prepared by the method has excellent lubricating property; the anti-inflammatory factor performance and the good biocompatibility are realized; the gel matrix prepared by the method can be well combined to the surface of damaged cartilage, overcomes the defects of insufficient mechanical property and poor anti-inflammatory factor property of single I-type collagen, is simple and convenient inpreparation method and easy in synthesis of raw materials, has good feasibility and practicability, and can be widely applied to articular cartilage repair.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a novel gel matrix based on dopamine-grafted zwitterionic polymerized phosphorylcholine-modified type I collagen and a preparation method thereof. The novel gel matrix of the present invention is especially suitable for chondrocyte culture, and has anti-nonspecific adsorption performance and lubricating performance at the same time. Background technique [0002] Arthritis is one of the most common chronic diseases. According to statistics, there are 355 million arthritis patients in the world, of which 190 million suffer from osteoarthritis and more than 16.5 million suffer from rheumatoid arthritis. Among them, arthritis caused by acute and chronic cartilage tissue damage is recognized as a disabling disease worldwide, accounting for about 30% of patients with arthritis. The treatment of this disease is a recognized problem internationally. Traditional surgical treatment m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C12N5/077C08L89/00
CPCC08J7/12C08J2343/02C08J2389/00C12N5/0655C12N2533/30C12N2533/54
Inventor 崔洪金姜坤妤陈明苏荣欣袁慧黄仁亮齐葳
Owner ARTHRO ANDA TIANJIN BIOLOGIC TECH
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