Joint injection preparation based on collagen-binding polypeptide modified hyaluronic acid and its preparation method and application

A hyaluronic acid and peptide-binding technology, which is applied in pharmaceutical formulations, prostheses, drug delivery, etc., can solve problems such as joint injection preparations not combining with damaged cartilage surfaces, multiple injections and repeated administration, etc., to achieve good resistance Non-specific adsorption performance, overcoming insufficient lubrication performance, good biocompatibility effect

Active Publication Date: 2021-10-22
天津永续新材料有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, no synthetic collagen-binding peptides have been found to modify hyaluronic acid, and then prepare joint injection preparations for the treatment of cartilage damage, so as to overcome the shortcomings of the existing joint injection preparations that cannot be combined with the damaged cartilage surface and require multiple injections and repeated administration. report

Method used

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  • Joint injection preparation based on collagen-binding polypeptide modified hyaluronic acid and its preparation method and application
  • Joint injection preparation based on collagen-binding polypeptide modified hyaluronic acid and its preparation method and application
  • Joint injection preparation based on collagen-binding polypeptide modified hyaluronic acid and its preparation method and application

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Embodiment 1

[0034] 1) Preparation of collagen-binding polypeptide-modified hyaluronic acid:

[0035] Prepare a 10 mmol / L phosphate buffer solution with pH=7.4 with disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate. Dissolve 1.2g of hyaluronic acid (HA) in 120mL of the above phosphate buffer, adjust the pH of the solution to 5 with 1.0mol / L hydrochloric acid, and then add 1-ethyl-(3-dimethylamino Propyl) carbodiimide hydrochloride (1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and hyaluronic acid repeating unit molar ratio is 1:1), after stirring for 20min Collagen-binding polypeptides were added (the molar ratio of collagen-binding polypeptides to hyaluronic acid repeating units was 1:1), the pH of the solution was maintained at 5 with 1 mol / L hydrochloric acid, and the reaction was stirred at 20° C. for 1 hour. The reacted solution was placed in a dialysis bag (MWCO: 8000-14000Da), and dialyzed in an aqueous solution with pH=5.0 for 2 days, and th...

Embodiment 2

[0053] 1) Preparation of collagen-binding polypeptide-modified hyaluronic acid:

[0054] Prepare a 10 mmol / L phosphate buffer solution with pH=7.4 with disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate. Dissolve 1.2g of hyaluronic acid (HA) in 120mL of the above phosphate buffer, adjust the pH of the solution to 5.5 with 1.0mol / L hydrochloric acid, and then add 1-ethyl-(3-dimethylamino Propyl) carbodiimide hydrochloride (1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and hyaluronic acid repeating unit molar ratio is 1:1), after stirring for 25min Collagen-binding polypeptides were added (the molar ratio of collagen-binding polypeptides to hyaluronic acid repeating units was 2:1), the pH of the solution was maintained at 5.5 with 1 mol / L hydrochloric acid, and the reaction was stirred at 25°C for 2 hours. The reacted solution was placed in a dialysis bag (MWCO: 8000-14000Da), and dialyzed in an aqueous solution with pH=5.0 for 2 days, and...

Embodiment 3

[0070] 1) Preparation of collagen-binding polypeptide-modified hyaluronic acid:

[0071] Prepare a 10 mmol / L phosphate buffer solution with pH=7.4 with disodium hydrogen phosphate dodecahydrate and potassium dihydrogen phosphate. Dissolve 1.2g of hyaluronic acid (HA) in 120mL of the above phosphate buffer, adjust the pH of the solution to 6 with 1.0mol / L hydrochloric acid, and then add 1-ethyl-(3-dimethylamino Propyl) carbodiimide hydrochloride (1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride and hyaluronic acid repeating unit molar ratio is 1:1), after stirring for 30min Collagen-binding polypeptides were added (the molar ratio of collagen-binding polypeptides to hyaluronic acid repeating units was 3:1), the pH of the solution was maintained at 6 with 1 mol / L hydrochloric acid, and the reaction was stirred at 30°C for 3 hours. The reacted solution was placed in a dialysis bag (MWCO: 8000-14000Da), and dialyzed in an aqueous solution with pH=5.0 for 2 days, and the...

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Abstract

The invention discloses a joint injection preparation based on collagen-binding polypeptide modified hyaluronic acid and its preparation method and application. Firstly, a polypeptide molecule capable of binding collagen is designed and synthesized, and then the hyaluronic acid is grafted and modified through amidation reaction. Hyaluronic acid, and then through the specific recognition and binding of collagen-binding polypeptides and type II collagen on the surface of cartilage, hyaluronic acid is embedded on the surface of cartilage matrix, thereby obtaining a joint based on collagen-binding polypeptide-modified hyaluronic acid Injectable formulations. The joint injection preparation can not only specifically bind hyaluronic acid on the surface of damaged cartilage tissue, but also endow the damaged cartilage surface with excellent non-specific adsorption resistance and lubricity, and can be used to prepare cartilage repair materials and treat bone joints Inflammation, improving anti-inflammatory factors and lubricating properties on the surface of damaged cartilage; it can also be used as a modified material to improve the friction properties of cartilage repair materials.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a joint injection preparation, in particular to a joint injection preparation based on collagen-binding polypeptide-modified hyaluronic acid, a preparation method and application thereof. Background technique [0002] Arthritis is one of the most common chronic diseases, and arthritis caused by acute and chronic cartilage tissue damage is recognized as a disabling disease in the world, and its incidence rate accounts for about 30% of patients with arthritis diseases, and its treatment has not yet been overcome world problems. The traditional surgical treatment methods for arthritis caused by acute and chronic cartilage tissue injury mainly include arthroscopic irrigation, microfracture, and arthrodesis. Although some potential drugs are being tested in clinical trials, they can only reduce the suffering of patients in the short term, but cannot fundamentally improve or treat os...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/50
CPCA61L27/20A61L27/24A61L27/50A61L2400/06A61L2400/10A61L2430/06C08L5/08
Inventor 苏荣欣黄仁亮袁慧崔美齐崴
Owner 天津永续新材料有限公司
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