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A system and method for culturing stem cells

A stem cell culture and stem cell technology, applied in the field of biomedical engineering, can solve problems such as stem cell damage, achieve the effects of reducing stacking, avoiding damage, and improving culture efficiency

Active Publication Date: 2022-04-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the three-dimensional stem cell culture process, some internal forces, such as magnetic force or vibration, are required to maintain the suspension state in the culture medium, and the resulting shear force will inevitably cause damage to the stem cells.

Method used

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  • A system and method for culturing stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: System for Stem Cell Culture

[0041] The system for stem cell culture, the schematic diagram of which is shown in figure 1 shown. The system includes:

[0042] Material supply device 1, described material supply device 1 is provided with material injection port 11, material outlet 12 and culture medium backflow inlet 15, is provided with the first bacteria filter membrane 13 in the material supply device 1, in described first bacteria filter membrane 13 is provided with an agitator 14; the substance outlet 12 is located below the first bacteria filter membrane 13, and the culture medium return inlet 15 is located above the first bacteria filter membrane 13.

[0043] Air communication device 2, the inlet 24 of the air communication device 2 communicates with the material outlet 12 of the material supply device 1 through the first connecting pipe 6, and the top of the air communication device 2 is provided with an air filter 21 and a carbon dioxide inlet 28 ...

Embodiment 2

[0048] Embodiment 2: three-dimensional culture of mesenchymal stem cells (MSC)

[0049] The system of Example 1 was used to carry out three-dimensional culture of human bone marrow mesenchymal stem cells, as follows:

[0050] (1) Inoculate the human bone marrow mesenchymal stem cells to be cultured onto the microcarriers 32 in the stem cell culture device 3; add the culture medium into the material supply device 1 through the material injection port 11, stir evenly with the agitator 14, and then Filter for the first time by the first bacteria filter membrane 13;

[0051] The composition of the medium is: α-MEM medium + 10% fetal bovine serum + 0.5% penicillin + 0.5% streptomycin + 2ng / ml basic fibroblast growth factor (bFGF).

[0052] (2) The culture medium after the first filter is transported from the material supply device 1 to the air communication device 2 through the first connecting pipe 6, and the air communication device 2 communicates with the outside air through th...

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Abstract

The invention discloses a system and a cultivation method for stem cell cultivation, belonging to the technical field of biomedical engineering. The system of the present invention includes: a material supply device, an air communication device, a stem cell culture device and a metabolism recovery device. By adopting the system and culture method of the present invention, stem cell activity can be avoided due to excessive shear force in the process of three-dimensional culture, while the efficiency of stem cell culture is improved as much as possible, and the multi-directional differentiation potential of stem cells is maintained.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a system and method for culturing stem cells. Background technique [0002] Stem cells are cells with multidirectional differentiation potential and self-renewal ability, which can differentiate into cells of a specific tissue type in vivo. Due to the relatively low immunogenicity of stem cells and their immunoregulatory effects, stem cells have become the most ideal seed cells for cell therapy and tissue and organ replacement therapy. Patients can use their own adult stem cells to treat their own diseases and avoid immune rejection. For example, MSC, which is the most popular in the field of stem cell research in recent years, can suppress immune rejection by suppressing cellular immunity, and is very suitable for clinical transplantation treatment, and is of great significance in the treatment of cardiovascular diseases, liver diseases, and immune diseases. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/02C12M1/42C12M1/38C12M1/34C12M1/12C12M1/04C12M1/02
CPCC12M25/16C12M27/02C12M29/00C12M29/04C12M29/18C12M29/26C12M35/04C12M37/02C12M41/12C12M41/26C12M47/10
Inventor 刘宏杨鸿儒桑元华甘宜梧刘锋段佳志孔颖何建龙郝敏袁文虎王书华王建军
Owner SHANDONG UNIV
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