Sheath fluid flow chamber suitable for millimeter-scale large particle carrying
A flow chamber and large particle technology, applied in the field of flow cytometry, can solve the problem of inability to meet the requirements of small animals, and achieve the effect of manual screening
Pending Publication Date: 2020-09-08
苏州赛宁光学科技有限公司
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Problems solved by technology
[0005] However, the above-mentioned sheath fluid flow chamber can only be used for the analysis of cell screening, and cannot meet the requirements of tiny animals.
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[0028] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0029] Such as figure 1 As shown, a sheath liquid flow chamber suitable for carrying millimeter-scale large particles is a single-side injection method, and the angle between the sheath liquid injection and the sample injection is 90°. By increasing the position of the sample injection outlet and the sheath liquid The distance from the injection position to reduce the impact of liquid flow turning.
[0030] Described sheath fluid flow chamber comprises negative pressure exhaust bubble hole 1, sample inlet 2, sheath fluid inlet 3, sheath flow generation region 4, sensor detection region 5, observation region 6 and sealed cavity 7;
[0031] The sheath liquid sampling port 3 is arranged on one side of the sample sampling port 2, and is arranged at 90° with it, and the negative pressure air bubble hole 1 is arranged on the side of the sample sampling port 2; the...
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Abstract
The invention belongs to the field of flow cytometry, and particularly relates to a sheath fluid flow chamber suitable for millimeter-scale large particle carrying. The sheath fluid flowing chamber comprises a negative-pressure bubble discharging hole, a sample inlet, a sheath fluid sample inlet, a sheath fluid generation area, a sensor detection area, an observation area and a sealed cavity; andthe nuclear flow width of the sheath fluid flow chamber is between 0.1 mm and 1mm by controlling the sheath fluid flow rate and the diameter of the detection area. According to the invention, the characteristic that the liquid in the laminar state in the hydrodynamics has laminar axial movement is utilized, so that the sample liquid is wrapped by the sheath liquid to realize zebra fish carrying; by controlling the sheath fluid flow rate and the diameter of the detection area, the invention is suitable for a sheath fluid flow chamber for large-particle carrying of 0.1 mm-1mm, a sample focusingpath is provided for further automatic observation and screening of samples, and therefore the tedious process of manual screening and the like is avoided.
Description
technical field [0001] The invention belongs to the field of flow cytometry, and in particular relates to a sheath fluid flow chamber suitable for carrying millimeter-scale large particles. Background technique [0002] Millimeter large particles such as zebrafish, gelatin, pollen. However, the screening of its samples is still in the process of manual screening at this stage. Taking zebrafish as an example, as a commonly used model animal, zebrafish has important applications in genetic development research and drug screening. [0003] In the existing designs of sheath fluid flow chambers, they are mainly designed for flow cytometers, and their narrow focal width cannot meet the requirements of tiny animals. A flow cytometer sheath fluid flow chamber, authorized notification number CN 204903352U reduces the generation of turbulent flow by optimizing the sheath fluid entry and injection method, making it easier to form laminar flow. For micro model animals, which are larg...
Claims
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Login to View More IPC IPC(8): G01N15/14
CPCG01N15/1404G01N15/14
Inventor 王浩金鑫杨光李辉
Owner 苏州赛宁光学科技有限公司