A method for simultaneous determination of seven pigments in eggs

An egg and pigment technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of single target and inability to qualitative and quantitative analysis of multiple pigment substances.

Active Publication Date: 2021-08-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The limitation of the above method is that the detection target is relatively single or less, and it is impossible to conduct qualitative and quantitative analysis of multiple pigment substances in egg yolk at the same time, so as to distinguish natural nutrients from artificially added pigments.

Method used

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  • A method for simultaneous determination of seven pigments in eggs
  • A method for simultaneous determination of seven pigments in eggs
  • A method for simultaneous determination of seven pigments in eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Magnetic DQTP / Fe 3 o 4 Preparation methods of nanomaterials

[0022] Place a 3 mL test tube containing 17 mg of 2,6-diaminoanthraquinone (DQ) in DMF in a water bath at 90°C. 10mg of 1,3,5-trialdehyde phloroglucinol (TP) was formulated with DMF to make 10mg / L, and 0.05mL of 6mol / L acetic acid solution was added to the solution of acetic acid, which was fully ultrasonicated, and then 1mL was slowly added dropwise to DQ with a pipette gun. In hot solution, the process is controlled within 1h. After the TP solution was completely added, the mouth of the test tube was plugged with degreased cotton, and the mixed solution continued to be bathed in water at 90°C for 3 hours. The resultant was soaked in deionized water and acetone for 24 hours to replace unreacted ligands, and dried in vacuum to obtain DQTP. 80mg FeCl 3 ·6H 2 O and 50 mg FeSO 4 ·7H 2 O was dispersed in 20 mL of pure water. Add 70mg of synthesized DQTP and ultrasonically disperse at 80°C to p...

Embodiment 2

[0023] Embodiment 2: A kind of pretreatment method of extracting 7 kinds of pigments in eggs

Embodiment 3

[0026] Example 3: A method for detecting the content of 7 pigments in eggs

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Abstract

The invention discloses a method for simultaneously measuring seven pigments in eggs, belonging to the technical field of food safety detection. The method is to adopt DQTP / Fe 3 o 4 Organic covalent framework structure and magnetic nanocomposite materials, as a dispersed magnetic solid-phase extraction adsorbent to extract pigments in eggs, and then use ultra-high performance liquid chromatography tandem mass spectrometry UPLC-MS / MS method combined with external standard method to determine the pigments in eggs Pigment content. The invention establishes a method for simultaneously measuring the content of riboflavin, lutein, canthaxanthin and Sudan red I, Sudan red II, Sudan red III and Sudan red IV in eggs. The detection limits of lutein, canthaxanthin, Sudan Ⅰ, Sudan Ⅱ, Sudan Ⅲ and Sudan Ⅳ were all 0.2ug / kg, and the detection limit of riboflavin was 0.2ug / kg. The recoveries of seven pigments ranged from 2 to 10ug / kg, and the recoveries were 80.2 to 106.7%, and the relative standard deviations (RSD) were 1.5 to 6.0%.

Description

technical field [0001] The invention relates to a method for simultaneously measuring seven pigments in eggs, belonging to the technical field of food safety detection. Background technique [0002] With the continuous improvement of living standards, people's pursuit of natural and organic ingredients is becoming a trend. Eggs produced by free-range chickens under natural conditions, commonly known as "native eggs" or "grass eggs", are favored, and the price is twice or even several times higher than ordinary eggs. Ordinary consumers mainly distinguish native eggs from ordinary eggs by the color of the yolk, thinking that native eggs are darker in color. The color of egg yolk comes from pigment substances such as riboflavin and lutein. Free-range chickens have more opportunities to eat leaves, seeds and other foods rich in lutein. Lutein is a natural carotenoid substance, which is enriched in egg yolk after metabolism, making it appear bright orange. Cage chickens mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/04G01N30/72G01N30/34G01N30/86G01N30/88
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8634G01N30/8679G01N30/88G01N2030/047G01N2030/062G01N2030/065G01N2030/146G01N2030/884
Inventor 杨成黄晴沈晓芳孙嵇成
Owner JIANGNAN UNIV
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