Acquisition method of biocontrol bacteria for preventing and treating root diseases of continuous cropping plants
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An acquisition method and technology of biocontrol bacteria, applied in the field of soil-borne disease prevention and control, to achieve the effects of improving rhizosphere colonization ability, enhancing antibacterial spectrum, and good disease control effect
Pending Publication Date: 2020-09-11
NANJING FORESTRY UNIV
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Problems solved by technology
At present, there is a lack of a scientific method for judging the rhizosphere biocontrol bacteria
Method used
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Embodiment 1
[0035] 1. Method
[0036] Select peanut continuous cropping soil and non-continuous cropping soil for testing;
[0037] Pot experiment to isolate and identify peanut rhizosphere bacteria;
[0038] Determine the 7 strains of bacteria missing in the rhizosphere of continuous cropping peanut;
[0039] Prepare 7 strains, 4 strains and 2 strains of bacterial combinations in the peanut rhizosphere;
[0040] Determination of the disease inhibitory activity of 7 strains, 4 strains and 2 bacterial strains in the peanut rhizosphere;
[0041] Determination of the disease inhibitory activity of 7 bacterial strains in the rhizosphere of peanut and continuous cropping peanut bacteria;
[0042] Root irrigation application of 7 bacterial strains in peanut rhizosphere;
[0043] Seed soaking was applied to peanut rhizosphere with 7 strains, 4 strains and 2 bacterial combinations.
[0044] 2. Test soil
[0045] The peanut experimental field that has been continuously cropped for more than ...
Embodiment 2
[0094] 1. Method
[0095] Select continuous cropping soil and non-continuous cropping soil for test cotton;
[0096] Pot experiments to isolate and identify cotton rhizosphere bacteria;
[0097] Determine the 7 strains of bacteria missing in the rhizosphere of continuous cropping cotton;
[0098] Prepare 7 strains, 4 strains and 2 strains of bacterial combinations in the cotton rhizosphere;
[0099] Determination of the disease inhibitory activity of 7 strains, 4 strains and 2 bacterial strains in the rhizosphere of cotton;
[0100] Determination of the disease inhibitory activity of 7 strains of bacterial combinations in cotton rhizosphere and continuous cropping cotton bacteria;
[0101] Root irrigation application of 7 bacterial strains in cotton rhizosphere.
[0102] 2. Test soil
[0103] The cotton experimental field that has been continuously cropped for 15 years is selected as the test soil, and the experimental field where corn, soybean and cotton have been croppe...
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Abstract
The invention provides an acquisition method of biocontrol bacteria for preventing and treating root diseases of continuous cropping plants, and belongs to the technical field of soil-borne disease prevention and treatment. The acquisition method comprises the following steps: acquiring rhizosphere soil of a continuous cropping plot and rhizosphere soil of a non-continuous cropping plot; separating and identifying flora types of continuous cropping soil and non-continuous cropping soil; comparing continuous cropping flora with non-continuous cropping flora, and screening out lost flora in thecontinuous cropping flora, namely biocontrol bacteria for preventing and treating the root diseases of the continuous cropping plants; and verifying the antibacterial activity of the biocontrol bacteria. The method provided by the invention can effectively obtain the biocontrol bacteria for preventing and treating the root diseases of the continuous cropping plants. The biocontrol bacteria obtained through the method can improve the rhizosphere colonization capacity of microorganisms and enhance the bacteriostasis spectrum, has a good disease prevention and treatment effect and provides support for effective research and development of effective antibacterial agents, and therefore the bottleneck that the biocontrol bacterium is difficult to commercially popularize is fundamentally broken through.
Description
technical field [0001] The invention relates to a method for obtaining biocontrol bacteria for preventing and controlling root diseases of continuous cropping plants, and belongs to the technical field of soil-borne disease prevention and control. Background technique [0002] Continuous cropping obstacle is a common phenomenon in many plants. It refers to the phenomenon of poor crop growth, occurrence of diseases and insect pests, and reduced yield after continuous cropping of the same plant or related plants, even if normal cultivation and management measures are adopted. Limited to the area of arable land and the development of agricultural industrialization, the phenomenon of intensive production of many crops is common, resulting in serious obstacles to continuous cropping. Continuous cropping and heavy cropping often lead to aggravation of soil-borne fungal diseases, among which root disease is the most serious disease in continuous cropping land, and it is an import...
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Application Information
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