NOVEL MULTI-ORGAN-CHIPS ESTABLISHING DIFFERENTIATION OF iPSC-DERIVED CELLS INTO ORGAN EQUIVALENTS

A technology of organelles and cells, applied in the field of new multi-organ chips

Pending Publication Date: 2020-09-15
TISSUSE GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the co-cultivation of different stem cell-derived organ precursors to create a multi-organ chip with numerous models mimicking organ functions, especially no co-cultivation of different induced pluripotent stem cells on microfluidic devices ( iPSC)-derived cells are used to generate organ equivalents reported

Method used

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  • NOVEL MULTI-ORGAN-CHIPS ESTABLISHING DIFFERENTIATION OF iPSC-DERIVED CELLS INTO ORGAN EQUIVALENTS
  • NOVEL MULTI-ORGAN-CHIPS ESTABLISHING DIFFERENTIATION OF iPSC-DERIVED CELLS INTO ORGAN EQUIVALENTS
  • NOVEL MULTI-ORGAN-CHIPS ESTABLISHING DIFFERENTIATION OF iPSC-DERIVED CELLS INTO ORGAN EQUIVALENTS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0279] Chip Design of ADME-N Chip

[0280] A chip design with constraints scaled down from human physiology is realized. Consider dimensional data as well as flow characteristics. The layout consists of two circuits (called "blood" and "urine") containing chambers for incorporation of the following organ equivalents: intestine, liver, kidney (divided into glomeruli and tubules) and nerve Meta organization (Figure 1). The first three tissues are used to complete the so called ADME profile (Absorption, Distribution, Metabolism, Excretion). The latter are additional tissues that complement the profile. Therefore, the chip is called ADME-N chip. One reservoir compartment per circuit allowed sampling of the supernatant (medium reservoirs 1 and 2). The medium is perfused through the microfluidic network by two built-in pneumatic micropumps (one for each circuit). The circuits overlap in two renal compartments separated by a porous membrane made of polycarbonate.

[0281] inte...

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Abstract

The present disclosure relates to novel multi-organ-chips establishing the differentiation of induced pluripotent stem cell (iPSC)-derived cells into organ equivalents on microfluidic devices and corresponding methods of generating organ equivalents. The present disclosure also relates to novel bioengineered tissue constructs mimicking organ barriers generated with iPSC-derived endothelial cells and / or organoids bioprinted in, and / or seeded on, a hydrogel. The present disclosure further relates to methods of bioengineering organ constructs comprising co-culturing iPSC-derived organ precursor cells and iPSC-derived fibroblasts and endothelial cells. The present disclosure specifically provides a microfluidic device comprising: (i) iPSC-derived hepatocyte precursor cells; (ii) iPSC-derived intestinal precursor cells; (iii) iPSC-derived renal tubular precursor cells; and (iv) iPSC-derived neuronal precursor cells; wherein the iPSC-derived precursor cells according to (i), (ii), (iii) and(iv) are differentiated from a single donor iPSC reprogrammed from a single type of somatic cell.

Description

technical field [0001] The present disclosure relates to the establishment of novel multi-organ chips on microfluidic devices for differentiating induced pluripotent stem cell (iPSC)-derived cells into organ equivalents, and corresponding methods for generating organ equivalents. The present disclosure also relates to novel bioengineered tissue constructs that mimic organ barriers generated with iPSC-derived endothelial cells and / or organelles bioprinted in and / or seeded on hydrogels. The disclosure further relates to methods of bioengineering organ constructs comprising co-cultivating iPSC-derived organ precursor cells and iPSC-derived fibroblasts and endothelial cells. The present disclosure specifically provides a microfluidic device comprising: (i) iPSC-derived hepatocyte precursor cells; (ii) iPSC-derived intestinal precursor cells; (iii) iPSC-derived renal tubular precursor cells; and (iv) ) iPSC-derived neuronal precursor cells; wherein the iPSC-derived precursor cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/00
CPCC12N5/0075C12N5/0671C12N5/0679C12N5/0684C12N5/0686C12N5/0697C12N2502/13C12N2502/14C12N2502/23C12N2502/256C12N2506/45C12N2531/00C12M23/16C12N5/00C12N5/0672C12N5/0619C12N5/0656C12N2502/28
Inventor U·马克思A·拉姆
Owner TISSUSE GMBH
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