Stem cell amnion dressing structure for wound surface healing, and preparation method of stem cell amnion dressing structure for wound surface healing

An amniotic membrane dressing and wound repair technology, applied in the fields of medical science, bandages, absorbent pads, etc., can solve the problems that the amniotic membrane is easily torn and damaged, increase the difficulty of preparation materials and equipment management, and achieve easy identification of positive and negative sides and simple preparation method. , the effect of overcoming application defects

Inactive Publication Date: 2020-10-20
洛阳巴库生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this structure is an auxiliary structure, and the amniotic membrane is only temporarily fixed on the culture dish. When in use, it is still necessary to use tweezers to clamp the fixing collar out. Due to the height and size of the fixing collar, when it is used after clipping out, it is still It is necessary to remove the amniotic membrane from the fixed collar and use it again. During this process, the amniotic membrane is still easy to be torn and damaged, and this structure is equivalent to a new special culture structure. When the amniotic membrane is cultured, it is quite Due to the introduction of devices with new complex structures, it increases the difficulty in the management of materials and devices

Method used

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  • Stem cell amnion dressing structure for wound surface healing, and preparation method of stem cell amnion dressing structure for wound surface healing
  • Stem cell amnion dressing structure for wound surface healing, and preparation method of stem cell amnion dressing structure for wound surface healing
  • Stem cell amnion dressing structure for wound surface healing, and preparation method of stem cell amnion dressing structure for wound surface healing

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Embodiment 1

[0028]A stem cell amniotic membrane dressing structure for wound repair, including a three-part structure, the first part structure is a layer of medical nylon cloth, the size of the medical nylon cloth is 4cm×4cm, and the second part structure is the same as the outer contour shape of the medical nylon cloth For the same decellularized amniotic membrane, the decellularized amniotic membrane is sutured on the nylon cloth with nylon thread, so that the nylon cloth and the amniotic membrane can be attached to the maximum extent, to ensure that the amniotic membrane is fully stretched, and to avoid the amniotic membrane curling during the culture process. The structure of the first part and the structure of the second part Together they constitute the decellularized amniotic membrane scaffold, and the third part is the umbilical cord mesenchymal stem cells, which are covered on the decellularized amniotic membrane.

[0029] A method for preparing a stem cell amniotic membrane dres...

Embodiment 2

[0036] There is no essential difference between this example and Example 1, except that in step a, the single cell suspension was mixed according to 3×10 5 Cells / ml were seeded in culture flasks at a density of 37°C in a serum-free medium, and passaged and expanded when the cells reached 70% confluency.

[0037] A stem cell amniotic membrane dressing structure for wound repair, including a three-part structure, the first part structure is a layer of medical nylon cloth, the size of the medical nylon cloth is 4cm×4cm, and the second part structure is the same as the outer contour shape of the medical nylon cloth For the same decellularized amniotic membrane, the decellularized amniotic membrane is sutured on the nylon cloth with nylon thread, so that the nylon cloth and the amniotic membrane can be attached to the maximum extent, to ensure that the amniotic membrane is fully stretched, and to avoid the amniotic membrane curling during the culture process. The structure of the fi...

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Abstract

The invention discloses a stem cell amnion dressing structure for wound surface healing, and a preparation method of the stem cell amnion dressing structure for wound surface healing. According to thestem cell amnion dressing structure for wound surface healing, a first-part structure is a layer of medical nylon cloth, a second-part structure is an accellular amnion which is the same as the medical nylon cloth in outline shape and size, the accellular amnion is sutured on the nylon cloth, and the first-part structure and the second-part structure jointly form an accellular amnion stent; and athird-part structure is umbilical cord mesenchymal stem cells which cover the accellular amnion. The preparation method of the stem cell amnion dressing structure for wound surface healing comprisesthe following steps of step a, obtaining mesenchymal stem cells; step b, preparing the accellular amnion stent; and step c, loading the mesenchymal stem cells on the accellular amnion stent so as to obtain the stem cell amnion dressing structure. The stem cell amnion dressing structure disclosed by the invention is crimp resistant, easy in identification of positive and negative surfaces and not liable to damage.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a stem cell amniotic membrane dressing structure for wound repair and a preparation method thereof. Background technique [0002] The research content of tissue engineering includes four aspects, including seed cells, biomaterials, methods and techniques for constructing tissues and organs, and clinical application of tissue engineering. Among them, seed cells are various types of cells used in tissue engineering to regenerate tissues and organs. Seed cells used in tissue engineering must have strong ability to divide and proliferate, strong secretory function, no or only very weak immune rejection, and continuous After subculture and subculture, no change in shape, function and genetic material occurs. [0003] Umbilical cord mesenchymal stem cells are derived from the discarded umbilical cord tissue after birth. It has a wide range of sources and is conv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/42A61L15/44
CPCA61L15/40A61L15/42A61L15/44A61L2300/412
Inventor 刘婧杨学义耿涛刘苗苗宋国英王晓谦张静宋丹
Owner 洛阳巴库生物科技有限公司
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