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Gene slopt7 involved in the regulation of tomato fruit size, lycopene and β-carotene and its application

A gene and tomato technology, applied in the biological field, achieve good application prospects, improve appearance and nutritional quality

Active Publication Date: 2021-03-19
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether OPT protein is directly involved in the regulation of fruit size and carotenoid content has not been reported yet.

Method used

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  • Gene slopt7 involved in the regulation of tomato fruit size, lycopene and β-carotene and its application
  • Gene slopt7 involved in the regulation of tomato fruit size, lycopene and β-carotene and its application
  • Gene slopt7 involved in the regulation of tomato fruit size, lycopene and β-carotene and its application

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Embodiment Construction

[0042] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0043] 1. Tomato SlOPT7 (Solyc08g082990) gene knockout target design

[0044] In the present invention, the gene editing technology based on CRISPR / Cas9 system is used to knock out the S1OPT7 gene. The basic vector pYLCRISPR / Cas9Pbui-H (Genbank: KR029109) was donated to the research group of Academician Liu Yaoguang of South China Agricultural University. The specific vector construction process refers to the protocol published by his laboratory (Ma X, Liu Y. CRISPR / Cas9-Based Multiplex Genome Editing in Monocot and Dicot Plants. [J]. Current protocols in molecular biology, 2016, 115 (1)). Among them, the online tool CRISPR-P v2.0 (http: / / crispr.hzau.edu.cn / CRISPR2 / ) was used for target design and off-target prediction, and the designed sgRNA structure was predicted using the online software RNAFolding program ...

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Abstract

The invention relates to the technical field of biology, in particular to an application of a tomato SlOPT7 gene to regulation and control of tomato fruit size, lycopene and beta-carotene content. Theinvention discloses the gene SlOPT7 capable of simultaneously regulating and controlling the size of tomato fruits and the content of lycopene and beta-carotene. The nucleotide sequence of the gene SlOPT7 is as shown in SEQ ID NO: 1. The invention further discloses two homozygous editing materials, namely a homozygous editing material opt7-1 and a homozygous editing material opt7-2. The gene edited tomatoes constructed by the invention do not contain exogenous gene segments, the fruits are enlarged, and the contents of lycopene and beta-carotene are increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of tomato S1OPT7 gene in regulating tomato fruit size, lycopene and β-carotene content. Background technique [0002] Tomato (Solanum lycopersicum) is an important vegetable crop in the world. The yield and quality of tomato fruit directly determine its competitiveness in the fresh fruit and processing market. Studying the molecular mechanism of tomato fruit quality has important guiding significance for breeding high-quality tomato varieties. In addition, tomato is a model plant for studying the development and ripening of fleshy fruits, so the study of the molecular regulation mechanism of tomato fruit ripening is also of great guiding significance to the genetic regulation of fruit quality in other crops. [0003] Fruit size is an important factor affecting tomato fruit yield and appearance. Through domestication and improvement, the weight of modern cultivated to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/08A01H6/82
CPCC07K14/415C12N15/8218C12N15/825C12N15/8261
Inventor 谭金娟邓志平周忠静冯寒骞
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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