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Medusa-snow lotus high output flavone cell line

A cell line, snow lotus technology, applied in the field of jellyfish snow lotus high-yielding flavonoid cell lines, can solve the problems of slow natural growth, destructive wild resources, damage to the ecological environment, etc., achieve stable yield and content, save expensive investment, and low pollution rate Effect

Inactive Publication Date: 2003-08-20
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
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Problems solved by technology

But because Saussurea jellyfish grows near the alpine snow line above 4000 meters above sea level, so the introduction and cultivation has not been successful so far, still adopts to dig wild Saussurea jellyfish to extract flavonoids at present, and the shortcoming of this method is: (1) Saussurea jellyfish is a perennial herbaceous plant, naturally Slow growth, collecting wild snow lotus for medicine has serious damage to wild resources, and also damages the ecological environment

Method used

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Experimental program
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Effect test

Embodiment

[0019] 1. Preparation of Saussurea jellyfish cell line

[0020] A. Collect Saussurea jellyfish as explants (such as leaves, flowers, diameters) to induce callus. The explants are first washed with tap water for several hours, then soaked and sterilized with various fungicides, and washed 5-6 times with sterile water , 2-3min each time, inoculate on 1 / 2MS medium with different types and concentrations of phytohormone combinations after disinfection, place at 25±10°C, and cultivate under light. After two weeks of culture, callus tissue can be formed at the incision site of the outer finger body.

[0021] B. The callus is subcultured on MS medium (optimum 25±1°C, cultured under light), subcultured once every 15 days, and repeatedly screened by visual method, A-01 cell line can be obtained by repeated subculture ( yellow line). through 60 The high-yielding cell line A-1 cell line was obtained after Co irradiation, and was preserved and subcultured once every 15 days.

[0022] ...

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Abstract

A medusa-type snow lotus cell line (CGMCC 0484.1) for generating flavone with high output is disclosed. Its cell growth speed can reach 40-45 g / L / month. The content of flavone compounds is 7-9 wt.% of culture medium dry weight.

Description

technical field [0001] The invention relates to a jellyfish Saussurea jellyfish high-yield flavonoid cell line. Background technique [0002] Jellyfish Snow Lotus (Saussurea medussa Maxim) is a traditional Chinese medicine in my country. It is used in the folks to treat rheumatoid arthritis, women's irregular menstruation, and retained placenta. Clinically, it is also used in the treatment of arteriosclerosis. Snow lotus contains a variety of active ingredients, mainly flavonoids. In addition, snow lotus can also be used in cosmetics and so on. But because Saussurea jellyfish grows near the alpine snow line above 4000 meters above sea level, so the introduction and cultivation has not been successful so far, still adopts to dig wild Saussurea jellyfish to extract flavonoids at present, and the shortcoming of this method is: (1) Saussurea jellyfish is a perennial herbaceous plant, naturally The growth is slow, and collecting wild snow lotus for medicine has serious damage ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04
Inventor 赵德修李茂寅叶和春邢建民赵敬芳吕东平
Owner INST OF BOTANY CHINESE ACAD OF SCI
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