Medusa-snow lotus cell system for generating flavone with high output

A technology of cell line and snow lotus, which is applied in the field of high-yield flavonoid cell line of jellyfish snow lotus, can solve the problems of destroying the ecological environment, slow natural growth, and destructiveness of wild resources, and achieve the effects of protecting the ecological environment, increasing yield, and stabilizing quality and yield

Inactive Publication Date: 2003-10-01
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology

But because Saussurea jellyfish grows near the alpine snow line above 4000 meters above sea level, so the introduction and cultivation has not been successful so far, still adopts to dig wild Saussurea jellyfish to extract flavonoids at pre...

Method used

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Experimental program
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Embodiment

[0019] 1. Preparation of Saussurea jellyfish cell line

[0020] A. Collect explants (such as leaves, flowers, and diameters) of jellyfish Saussurea jellyfish to induce callus tissue. The explants are first washed with tap water for several hours, then soaked and sterilized with various fungicides, and washed 5-6 times with sterile water , 2-3min each time, inoculate on 1 / 2MS medium with different types and concentrations of phytohormone combinations after disinfection, place at 25±10°C, and cultivate under light. After two weeks of culture, callus tissue can be formed at the incision site of the outer finger body.

[0021] B. The callus is subcultured on MS medium (optimum 25±1°C, cultured under light), subcultured once every 15 days, and repeatedly screened by visual method, A-02 cell line can be obtained by repeated subculture ( red line). through 60 The high-yield cell line A-2 cell line was obtained after Co irradiation, and was preserved and subcultured once every 15 d...

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Abstract

The invention provides a jellyfish Saussurea jellyfish high-yield flavonoid cell line, and its preservation number is CGMCC0484.1. With the A-2 line and medium provided by the invention, the cell growth rate can reach 35-40 g dry weight/liter/month, and the flavonoid content can reach 6-7% of the culture dry weight.

Description

technical field [0001] The invention relates to a jellyfish Saussurea jellyfish high-yield flavonoid cell line. Background technique [0002] Jellyfish Snow Lotus (Saussurea medussa Maxim) is a traditional Chinese medicine in my country. It is used in the folks to treat rheumatoid arthritis, women's irregular menstruation, and retained placenta. Clinically, it is also used in the treatment of arteriosclerosis. Snow lotus contains a variety of active ingredients, mainly flavonoids. In addition, snow lotus can also be used in cosmetics and so on. But because Saussurea jellyfish grows near the alpine snow line above 4000 meters above sea level, so the introduction and cultivation has not been successful so far, still adopts to dig wild Saussurea jellyfish to extract flavonoids at present, and the shortcoming of this method is: (1) Saussurea jellyfish is a perennial herbaceous plant, naturally The growth is slow, and collecting wild snow lotus for medicine has serious damage ...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 赵德修邢建民叶和春李茂寅赵敬芳吕东平
Owner INST OF BOTANY CHINESE ACAD OF SCI
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