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Multi-dimensional chromatography fluorescence microscopic imaging system and method based on polarization structured light modulation

A technology of microscopic imaging and structured light, which is applied in the direction of microscopes, optics, optical components, etc., can solve the problems of reducing imaging speed and so on

Active Publication Date: 2020-11-06
北京艾锐精仪科技有限公司
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Problems solved by technology

One of the solutions is to use fluorescent confocal microscopy, but this technique requires point scanning t

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  • Multi-dimensional chromatography fluorescence microscopic imaging system and method based on polarization structured light modulation
  • Multi-dimensional chromatography fluorescence microscopic imaging system and method based on polarization structured light modulation
  • Multi-dimensional chromatography fluorescence microscopic imaging system and method based on polarization structured light modulation

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Embodiment Construction

[0065] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

[0066] Such as figure 1As shown, the multidimensional tomographic fluorescence microscopy imaging system based on polarization structured light modulation in this embodiment includes: two lasers Ls1 and Ls2, a beam combiner, an acousto-optic tunable filter AOTF, a beam expander, and a polarization beam splitter PBS , half-wave plate HWP, spatial light modulator SLM, condenser lens L3, spatial filter Mk, vortex half-wave plate VHWP, first dichroic mirror DM1, first 4f system, second dichroic mirror DM2 , objective lens OB, emission filter EF, lens tube lens TL, second 4f system, aperture AP, band splitting device and camera C; wherein, each laser emits a linearly polarized laser of a band, and two lasers Ls1 and Ls2 Separately emit linearly polarized lasers of different wavelength bands; the first beam is reflected by the first mirror M1 a...

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Abstract

The invention discloses a multi-dimensional chromatography fluorescence microscopic imaging system and method based on polarization structured light modulation. According to the invention, for illumination in each polarization modulation direction, two sinusoidal structured light illumination patterns with complementary phases are collected and the two sinusoidal structured light illumination patterns are averaged to obtain a uniform light illumination pattern; the uniform light illumination pattern is combined with one of the sinusoidal structured light illumination patterns, and an optical tomographic image is solved by using a HiLo algorithm; only the three structured light patterns need to be averaged to obtain a uniform light illumination pattern, and then the uniform light illumination pattern is combined with one of the structured light illumination patterns, so that the optical tomography pattern can be solved by utilizing a HiLo algorithm. According to the invention, a detection end light splitting and collection method is adopted, and extra collection time does not need to be increased; the system and method have great advantages in the aspects of imaging speed, less photobleaching and the like.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to a multi-dimensional tomographic fluorescence microscopic imaging system and method based on polarization structured light modulation. Background technique [0002] Fluorescence microscopy imaging can perform non-invasive and specific observation of biological samples, which is of great significance in the research of subcellular structure and function. The basic physical properties of fluorescence include fluorescence intensity, polarization, spectrum, and fluorescence lifetime. Detecting these physical properties as much as possible can obtain more diverse information inside the cell. The method proposed by the invention can detect multidimensional information such as fluorescence intensity, spectrum and polarization. The present invention adopts polarization modulation illumination at the illumination end, through the modulation illumination of no less than three pol...

Claims

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Application Information

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IPC IPC(8): G02B21/00G02B27/00
CPCG02B21/0076G02B27/0012
Inventor 席鹏张昊李美琪刘文辉戴琼海
Owner 北京艾锐精仪科技有限公司
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